BD PharmingenTM PE Rat Anti-Mouse CD368 (Clec4d)

Description

Multiparameter flow cytometric analysis of CD368 Clec4d expression on BALB/c Mouse thioglycolate-elicited peritoneal exudate cells Thio-PECs . BALB/c Mouse Thioglycolate-elicited peritoneal cells were stained with BD Pharmingen APC Rat Anti-Mouse Ly-6G antibody Cat. No.560599 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD368 Clec4d antibody Cat. No. 571181/571182; Right Plot at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD368 Clec4d or Ig Isotype control staining versus Ly-6G was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD368 Clec4d expression on BALB/c Mouse thioglycolate-elicited peritoneal exudate cells Thio-PECs . BALB/c Mouse Thioglycolate-elicited peritoneal cells were stained with BD Pharmingen APC Rat Anti-Mouse Ly-6G antibody Cat. No.560599 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD368 Clec4d antibody Cat. No. 571181/571182; Right Plot at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD368 Clec4d or Ig Isotype control staining versus Ly-6G was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD368 Clec4d expression on BALB/c Mouse thioglycolate-elicited peritoneal exudate cells Thio-PECs . BALB/c Mouse Thioglycolate-elicited peritoneal cells were stained with BD Pharmingen APC Rat Anti-Mouse Ly-6G antibody Cat. No.560599 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD368 Clec4d antibody Cat. No. 571181/571182; Right Plot at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD368 Clec4d or Ig Isotype control staining versus Ly-6G was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD368 Clec4d expression on BALB/c Mouse thioglycolate-elicited peritoneal exudate cells Thio-PECs . BALB/c Mouse Thioglycolate-elicited peritoneal cells were stained with BD Pharmingen APC Rat Anti-Mouse Ly-6G antibody Cat. No.560599 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD368 Clec4d antibody Cat. No. 571181/571182; Right Plot at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD368 Clec4d or Ig Isotype control staining versus Ly-6G was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD368 Clec4d expression on BALB/c Mouse thioglycolate-elicited peritoneal exudate cells Thio-PECs . BALB/c Mouse Thioglycolate-elicited peritoneal cells were stained with BD Pharmingen APC Rat Anti-Mouse Ly-6G antibody Cat. No.560599 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD368 Clec4d antibody Cat. No. 571181/571182; Right Plot at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD368 Clec4d or Ig Isotype control staining versus Ly-6G was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD368 Clec4d expression on BALB/c Mouse thioglycolate-elicited peritoneal exudate cells Thio-PECs . BALB/c Mouse Thioglycolate-elicited peritoneal cells were stained with BD Pharmingen APC Rat Anti-Mouse Ly-6G antibody Cat. No.560599 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD368 Clec4d antibody Cat. No. 571181/571182; Right Plot at 1.0 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD368 Clec4d or Ig Isotype control staining versus Ly-6G was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative peritoneal cell populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.