BD PharmingenTM PE Rat Anti-Mouse CD73

Description

Two-color flow cytometric analysis of CD73 expression on splenic leucocytes from two mouse strains. C57BL/6 Top Plots and BALB/C Bottom Plots mouse splenic leucocytes were stained with APC Armenian Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plots or PE Rat Anti-Mouse CD73 Cat. No. 567215; Right Plots at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plots showing the correlated expression of CD73 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable DAPI- lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD73 expression on splenic leucocytes from two mouse strains. C57BL/6 Top Plots and BALB/C Bottom Plots mouse splenic leucocytes were stained with APC Armenian Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plots or PE Rat Anti-Mouse CD73 Cat. No. 567215; Right Plots at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plots showing the correlated expression of CD73 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable DAPI- lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD73 expression on splenic leucocytes from two mouse strains. C57BL/6 Top Plots and BALB/C Bottom Plots mouse splenic leucocytes were stained with APC Armenian Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plots or PE Rat Anti-Mouse CD73 Cat. No. 567215; Right Plots at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plots showing the correlated expression of CD73 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable DAPI- lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.