BD PharmingenTM PE Rat Anti-Mouse CD8b

Description

Two-color flow cytometric expression of CD8b expression on Mouse splenic lymphocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD8b antibody Cat. No. 568906/568907; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8b or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System equipped with a Yellow-Green Laser and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric expression of CD8b expression on Mouse splenic lymphocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD8b antibody Cat. No. 568906/568907; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8b or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System equipped with a Yellow-Green Laser and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric expression of CD8b expression on Mouse splenic lymphocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD8b antibody Cat. No. 568906/568907; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8b or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System equipped with a Yellow-Green Laser and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric expression of CD8b expression on Mouse splenic lymphocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD8b antibody Cat. No. 568906/568907; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8b or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System equipped with a Yellow-Green Laser and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric expression of CD8b expression on Mouse splenic lymphocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD8b antibody Cat. No. 568906/568907; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8b or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System equipped with a Yellow-Green Laser and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric expression of CD8b expression on Mouse splenic lymphocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse CD8b antibody Cat. No. 568906/568907; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8b or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System equipped with a Yellow-Green Laser and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.