BD PharmingenTM PE Rat Anti-Mouse CXCL16

Description

Two-color flow cytometric analysis of CXCL16 expression in mouse splenocytes. C57BL/6 mouse splenic leucocytes were cultured in complete tissue culture medium with BD GolgiPlug Protein Transport Inhibitor containing Brefeldin A Cat. No. 555029; 5h, 37C . The cell were harvested, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , BD Horizon BUV395 Rat Anti-Mouse CD19 Cat. No. 563557/565965 , APC Mouse Anti-Mouse NK1.1 antibody Cat. No. 550627/561117 , BD Horizon BV421 Rat Anti-Mouse CD11b Cat. No. 562605 antibodies and either PE Rat IgG1, kappa Isotype Control Cat. No. 554685; Left Plot or PE Rat Anti-Mouse CXCL16 antibody Cat. No. 566740; Right Plot at 0.5 mug /test. The two-color dot plot showing the correlated expression of CXCL16 or Ig Isotype control staining versus CD11b was derived from CD3- CD19- NK1.1- gated events with the forward and side light-scatter characteristics of intact splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CXCL16 expression in mouse RAW264.7 cells. Cells from the RAW 264.7 Macrophage ATCC TIB-71 cell line were cultured in complete tissue culture medium with BD GolgiPlug Protein Transport Inhibitor 5h, 37C . The cells were harvested, fixed with BD Cytofix Fixation Buffer, and then permeabilized with BD Perm/Wash Buffer and stained with either PE Rat IgG1, kappa Isotype Control dashed line histogram or PE Rat Anti-Mouse CXCL16 antibody solid line histogram at 0.5 mug/test. The fluorescence histogram showing CXCL16 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were similarly performed and the data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CXCL16 expression in mouse splenocytes. C57BL/6 mouse splenic leucocytes were cultured in complete tissue culture medium with BD GolgiPlug Protein Transport Inhibitor containing Brefeldin A Cat. No. 555029; 5h, 37C . The cell were harvested, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , BD Horizon BUV395 Rat Anti-Mouse CD19 Cat. No. 563557/565965 , APC Mouse Anti-Mouse NK1.1 antibody Cat. No. 550627/561117 , BD Horizon BV421 Rat Anti-Mouse CD11b Cat. No. 562605 antibodies and either PE Rat IgG1, kappa Isotype Control Cat. No. 554685; Left Plot or PE Rat Anti-Mouse CXCL16 antibody Cat. No. 566740; Right Plot at 0.5 mug /test. The two-color dot plot showing the correlated expression of CXCL16 or Ig Isotype control staining versus CD11b was derived from CD3- CD19- NK1.1- gated events with the forward and side light-scatter characteristics of intact splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CXCL16 expression in mouse RAW264.7 cells. Cells from the RAW 264.7 Macrophage ATCC TIB-71 cell line were cultured in complete tissue culture medium with BD GolgiPlug Protein Transport Inhibitor 5h, 37C . The cells were harvested, fixed with BD Cytofix Fixation Buffer, and then permeabilized with BD Perm/Wash Buffer and stained with either PE Rat IgG1, kappa Isotype Control dashed line histogram or PE Rat Anti-Mouse CXCL16 antibody solid line histogram at 0.5 mug/test. The fluorescence histogram showing CXCL16 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were similarly performed and the data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CXCL16 expression in mouse splenocytes. C57BL/6 mouse splenic leucocytes were cultured in complete tissue culture medium with BD GolgiPlug Protein Transport Inhibitor containing Brefeldin A Cat. No. 555029; 5h, 37C . The cell were harvested, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062/553061/561827 , BD Horizon BUV395 Rat Anti-Mouse CD19 Cat. No. 563557/565965 , APC Mouse Anti-Mouse NK1.1 antibody Cat. No. 550627/561117 , BD Horizon BV421 Rat Anti-Mouse CD11b Cat. No. 562605 antibodies and either PE Rat IgG1, kappa Isotype Control Cat. No. 554685; Left Plot or PE Rat Anti-Mouse CXCL16 antibody Cat. No. 566740; Right Plot at 0.5 mug /test. The two-color dot plot showing the correlated expression of CXCL16 or Ig Isotype control staining versus CD11b was derived from CD3- CD19- NK1.1- gated events with the forward and side light-scatter characteristics of intact splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CXCL16 expression in mouse RAW264.7 cells. Cells from the RAW 264.7 Macrophage ATCC TIB-71 cell line were cultured in complete tissue culture medium with BD GolgiPlug Protein Transport Inhibitor 5h, 37C . The cells were harvested, fixed with BD Cytofix Fixation Buffer, and then permeabilized with BD Perm/Wash Buffer and stained with either PE Rat IgG1, kappa Isotype Control dashed line histogram or PE Rat Anti-Mouse CXCL16 antibody solid line histogram at 0.5 mug/test. The fluorescence histogram showing CXCL16 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were similarly performed and the data shown on this Technical Data Sheet are not lot specific.