BD PharmingenTM PE Rat Anti-Mouse DCAR2 (Clec4b1)

Description

Multi-color flow cytometric analysis of DCAR2 Clec4b1 expression on mouse leucocytes and transfectants. Left and Middle Panels: Bone marrow cells from C57BL/6 mice were incubated in the presence of Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 or 553142 and were stained simultaneously with APC Hamster Anti-Mouse CD11c Cat. No. 550261 or 561119 and FITC Rat Anti-Mouse I-A/I-E antibody Cat. No. 553623 or 562009 to gate on conventional dendritic cells cDCs as shown in the left panel. At the same time, the samples of the bone marrow cells were also stained with either PE Rat Anti-Mouse DCAR2 Clec4b1 Cat. No. 566882 or PE Rat IgG2b, kappa Isotype Control Cat. No. 553989 at 0.5 mug/test. The overlayed plots of the middle panel were derived from gated cDCs showing expression of DCAR2 Clec4b1 or Ig Isotype Control staining solid and dashed line histograms, respectively . Right Panel: Non-transfected dotted line histogram and mouse DCAR2-transfected solid line histogram 293F cells were surface stained with PE Rat Anti-Mouse DCAR2 Clec4b1 antibody Cat. No. 566882 at 0.5 mug/test. Histogram plots showing expression of DCAR2 Clec4b1 on non-transfected or Mouse DCAR2-transfected 293F cells were derived from gated events with forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multi-color flow cytometric analysis of DCAR2 Clec4b1 expression on mouse leucocytes and transfectants. Left and Middle Panels: Bone marrow cells from C57BL/6 mice were incubated in the presence of Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 or 553142 and were stained simultaneously with APC Hamster Anti-Mouse CD11c Cat. No. 550261 or 561119 and FITC Rat Anti-Mouse I-A/I-E antibody Cat. No. 553623 or 562009 to gate on conventional dendritic cells cDCs as shown in the left panel. At the same time, the samples of the bone marrow cells were also stained with either PE Rat Anti-Mouse DCAR2 Clec4b1 Cat. No. 566882 or PE Rat IgG2b, kappa Isotype Control Cat. No. 553989 at 0.5 mug/test. The overlayed plots of the middle panel were derived from gated cDCs showing expression of DCAR2 Clec4b1 or Ig Isotype Control staining solid and dashed line histograms, respectively . Right Panel: Non-transfected dotted line histogram and mouse DCAR2-transfected solid line histogram 293F cells were surface stained with PE Rat Anti-Mouse DCAR2 Clec4b1 antibody Cat. No. 566882 at 0.5 mug/test. Histogram plots showing expression of DCAR2 Clec4b1 on non-transfected or Mouse DCAR2-transfected 293F cells were derived from gated events with forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multi-color flow cytometric analysis of DCAR2 Clec4b1 expression on mouse leucocytes and transfectants. Left and Middle Panels: Bone marrow cells from C57BL/6 mice were incubated in the presence of Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 or 553142 and were stained simultaneously with APC Hamster Anti-Mouse CD11c Cat. No. 550261 or 561119 and FITC Rat Anti-Mouse I-A/I-E antibody Cat. No. 553623 or 562009 to gate on conventional dendritic cells cDCs as shown in the left panel. At the same time, the samples of the bone marrow cells were also stained with either PE Rat Anti-Mouse DCAR2 Clec4b1 Cat. No. 566882 or PE Rat IgG2b, kappa Isotype Control Cat. No. 553989 at 0.5 mug/test. The overlayed plots of the middle panel were derived from gated cDCs showing expression of DCAR2 Clec4b1 or Ig Isotype Control staining solid and dashed line histograms, respectively . Right Panel: Non-transfected dotted line histogram and mouse DCAR2-transfected solid line histogram 293F cells were surface stained with PE Rat Anti-Mouse DCAR2 Clec4b1 antibody Cat. No. 566882 at 0.5 mug/test. Histogram plots showing expression of DCAR2 Clec4b1 on non-transfected or Mouse DCAR2-transfected 293F cells were derived from gated events with forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.