BD PharmingenTM PE Rat Anti-Mouse DCIR4 (Clec4a1)

Description

Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-CD11b antibody Cat. No. 562605 and either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 567034; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-CD11b antibody Cat. No. 562605 and either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 567034; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-CD11b antibody Cat. No. 562605 and either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 567034; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-CD11b antibody Cat. No. 562605 and either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 567034; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-CD11b antibody Cat. No. 562605 and either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 567034; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-CD11b antibody Cat. No. 562605 and either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 567034; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.