BD PharmingenTM PE Rat Anti-Mouse F4/80

Description

Two-color flow cytometric analysis of F4/80 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse CD11b Cat. No. 557686 and either PE Rat IgG2a Isotype Control Cat. No. 553930; Left Panel or PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410; Right Panel . The two-color flow cytometric contour plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of F4/80 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse CD11b Cat. No. 557686 and either PE Rat IgG2a Isotype Control Cat. No. 553930; Left Panel or PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410; Right Panel . The two-color flow cytometric contour plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of F4/80 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse CD11b Cat. No. 557686 and either PE Rat IgG2a Isotype Control Cat. No. 553930; Left Panel or PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410; Right Panel . The two-color flow cytometric contour plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of F4/80 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse CD11b Cat. No. 557686 and either PE Rat IgG2a Isotype Control Cat. No. 553930; Left Panel or PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410; Right Panel . The two-color flow cytometric contour plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Two-color flow cytometric analysis of F4/80 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse CD11b Cat. No. 557686 and either PE Rat IgG2a Isotype Control Cat. No. 553930; Left Panel or PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410; Right Panel . The two-color flow cytometric contour plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Two-color flow cytometric analysis of F4/80 expression on mouse splenic leucocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse CD11b Cat. No. 557686 and either PE Rat IgG2a Isotype Control Cat. No. 553930; Left Panel or PE Rat Anti-Mouse F4/80 antibody Cat. No. 565410; Right Panel . The two-color flow cytometric contour plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.