BD PharmingenTM PE Rat Anti-Mouse H-2 Class I

Description

Flow cytometric analysis of H-2 Class I expression on mouse splenocytes or thymocytes. C57BL/6 mouse splenic leucocytes Left Plot or thymocytes Right Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either PE Rat IgG2a, kappa Isotype Control Cat. No.557229; dashed line histograms or PE Mouse Anti-H-2 Class I antibody Cat. No. 566776; solid line histograms at 0.25 mug/test. The fluorescence histograms showing H-2 Class I expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of H-2 Class I expression on mouse splenocytes or thymocytes. C57BL/6 mouse splenic leucocytes Left Plot or thymocytes Right Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either PE Rat IgG2a, kappa Isotype Control Cat. No.557229; dashed line histograms or PE Mouse Anti-H-2 Class I antibody Cat. No. 566776; solid line histograms at 0.25 mug/test. The fluorescence histograms showing H-2 Class I expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of H-2 Class I expression on mouse splenocytes or thymocytes. C57BL/6 mouse splenic leucocytes Left Plot or thymocytes Right Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either PE Rat IgG2a, kappa Isotype Control Cat. No.557229; dashed line histograms or PE Mouse Anti-H-2 Class I antibody Cat. No. 566776; solid line histograms at 0.25 mug/test. The fluorescence histograms showing H-2 Class I expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of H-2 Class I expression on mouse splenocytes or thymocytes. C57BL/6 mouse splenic leucocytes Left Plot or thymocytes Right Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either PE Rat IgG2a, kappa Isotype Control Cat. No.557229; dashed line histograms or PE Mouse Anti-H-2 Class I antibody Cat. No. 566776; solid line histograms at 0.25 mug/test. The fluorescence histograms showing H-2 Class I expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of H-2 Class I expression on mouse splenocytes or thymocytes. C57BL/6 mouse splenic leucocytes Left Plot or thymocytes Right Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either PE Rat IgG2a, kappa Isotype Control Cat. No.557229; dashed line histograms or PE Mouse Anti-H-2 Class I antibody Cat. No. 566776; solid line histograms at 0.25 mug/test. The fluorescence histograms showing H-2 Class I expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of H-2 Class I expression on mouse splenocytes or thymocytes. C57BL/6 mouse splenic leucocytes Left Plot or thymocytes Right Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either PE Rat IgG2a, kappa Isotype Control Cat. No.557229; dashed line histograms or PE Mouse Anti-H-2 Class I antibody Cat. No. 566776; solid line histograms at 0.25 mug/test. The fluorescence histograms showing H-2 Class I expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.