BD PharmingenTM PE Rat Anti-Mouse IgG1

Description

Flow cytometric analysis of CD22.2 expression by mouse splenocytes using PE Rat Anti-Mouse IgG1 as a second step. BALB/c mouse splenic leucocytes were stained with either Purified Mouse IgG1, kappa Anti-Mouse CD22.2 antibody clone Cy34.1; solid line histogram or with no antibody for background staining control; dashed line histogram . After washing, the cells were stained with PE Rat Anti-Mouse IgG1 antibody Cat. No. 550083 as the second step at 1 mug/test. The fluorescence histogram showing CD22.2 expression or control staining as detected by PE Rat Anti-Mouse IgG1 antibody was derived from gated events with the forward and side light-scatter characteristics of intact splenocytes. Flow cytometry and analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Flow cytometric analysis of CD22.2 expression by mouse splenocytes using PE Rat Anti-Mouse IgG1 as a second step. BALB/c mouse splenic leucocytes were stained with either Purified Mouse IgG1, kappa Anti-Mouse CD22.2 antibody clone Cy34.1; solid line histogram or with no antibody for background staining control; dashed line histogram . After washing, the cells were stained with PE Rat Anti-Mouse IgG1 antibody Cat. No. 550083 as the second step at 1 mug/test. The fluorescence histogram showing CD22.2 expression or control staining as detected by PE Rat Anti-Mouse IgG1 antibody was derived from gated events with the forward and side light-scatter characteristics of intact splenocytes. Flow cytometry and analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Show More Flow cytometric analysis of CD22.2 expression by mouse splenocytes using PE Rat Anti-Mouse IgG1 as a second step. BALB/c mouse splenic leucocytes were stained with either Purified Mouse IgG1, kappa Anti-Mouse CD22.2 antibody clone Cy34.1; solid line histogram or with no antibody for background staining control; dashed line histogram . After washing, the cells were stained with PE Rat Anti-Mouse IgG1 antibody Cat. No. 550083 as the second step at 1 mug/test. The fluorescence histogram showing CD22.2 expression or control staining as detected by PE Rat Anti-Mouse IgG1 antibody was derived from gated events with the forward and side light-scatter characteristics of intact splenocytes. Flow cytometry and analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software.