BD PharmingenTM PE Rat Anti-Mouse IL-17A

Description

Characterization of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were cultured overnight in the presence of GolgiPlug 1 mug/ml final concentration; Cat. No.555029 without left panel or with PMA 5 ng/ml final concentration; Sigma, Cat. No.P-8139 and Ionomycin 500 ng/ml final concentration; Sigma, Cat. No.I-0634 middle and right panels . The cells were fixed, permeabilized and subsequently stained with 0.125 mug of PE conjugated anti-mouse IL-17A PE-TC11-18H10, Cat. No.559502 using Pharmingens I/C staining protocol left and middle panels . To demonstrate the specificity of staining, the binding of PE-TC11-18H10 was blocked by the preincubation of the fixed/permeabilized, stimulated EL4 cells with unlabeled TC11-18H10 antibody 10 mug; Cat. No. 559501, right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the unstimulated stained left panel and unlabeled antibody blocking right panel controls. Characterization of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were cultured overnight in the presence of GolgiPlug 1 mug/ml final concentration; Cat. No.555029 without left panel or with PMA 5 ng/ml final concentration; Sigma, Cat. No.P-8139 and Ionomycin 500 ng/ml final concentration; Sigma, Cat. No.I-0634 middle and right panels . The cells were fixed, permeabilized and subsequently stained with 0.125 mug of PE conjugated anti-mouse IL-17A PE-TC11-18H10, Cat. No.559502 using Pharmingens I/C staining protocol left and middle panels . To demonstrate the specificity of staining, the binding of PE-TC11-18H10 was blocked by the preincubation of the fixed/permeabilized, stimulated EL4 cells with unlabeled TC11-18H10 antibody 10 mug; Cat. No. 559501, right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the unstimulated stained left panel and unlabeled antibody blocking right panel controls. Characterization of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were cultured overnight in the presence of GolgiPlug 1 mug/ml final concentration; Cat. No.555029 without left panel or with PMA 5 ng/ml final concentration; Sigma, Cat. No.P-8139 and Ionomycin 500 ng/ml final concentration; Sigma, Cat. No.I-0634 middle and right panels . The cells were fixed, permeabilized and subsequently stained with 0.125 mug of PE conjugated anti-mouse IL-17A PE-TC11-18H10, Cat. No.559502 using Pharmingens I/C staining protocol left and middle panels . To demonstrate the specificity of staining, the binding of PE-TC11-18H10 was blocked by the preincubation of the fixed/permeabilized, stimulated EL4 cells with unlabeled TC11-18H10 antibody 10 mug; Cat. No. 559501, right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the unstimulated stained left panel and unlabeled antibody blocking right panel controls. Show More Characterization of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were cultured overnight in the presence of GolgiPlug 1 mug/ml final concentration; Cat. No.555029 without left panel or with PMA 5 ng/ml final concentration; Sigma, Cat. No.P-8139 and Ionomycin 500 ng/ml final concentration; Sigma, Cat. No.I-0634 middle and right panels . The cells were fixed, permeabilized and subsequently stained with 0.125 mug of PE conjugated anti-mouse IL-17A PE-TC11-18H10, Cat. No.559502 using Pharmingens I/C staining protocol left and middle panels . To demonstrate the specificity of staining, the binding of PE-TC11-18H10 was blocked by the preincubation of the fixed/permeabilized, stimulated EL4 cells with unlabeled TC11-18H10 antibody 10 mug; Cat. No. 559501, right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the unstimulated stained left panel and unlabeled antibody blocking right panel controls. Show More Characterization of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were cultured overnight in the presence of GolgiPlug 1 mug/ml final concentration; Cat. No.555029 without left panel or with PMA 5 ng/ml final concentration; Sigma, Cat. No.P-8139 and Ionomycin 500 ng/ml final concentration; Sigma, Cat. No.I-0634 middle and right panels . The cells were fixed, permeabilized and subsequently stained with 0.125 mug of PE conjugated anti-mouse IL-17A PE-TC11-18H10, Cat. No.559502 using Pharmingens I/C staining protocol left and middle panels . To demonstrate the specificity of staining, the binding of PE-TC11-18H10 was blocked by the preincubation of the fixed/permeabilized, stimulated EL4 cells with unlabeled TC11-18H10 antibody 10 mug; Cat. No. 559501, right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the unstimulated stained left panel and unlabeled antibody blocking right panel controls. Characterization of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were cultured overnight in the presence of GolgiPlug 1 mug/ml final concentration; Cat. No.555029 without left panel or with PMA 5 ng/ml final concentration; Sigma, Cat. No.P-8139 and Ionomycin 500 ng/ml final concentration; Sigma, Cat. No.I-0634 middle and right panels . The cells were fixed, permeabilized and subsequently stained with 0.125 mug of PE conjugated anti-mouse IL-17A PE-TC11-18H10, Cat. No.559502 using Pharmingens I/C staining protocol left and middle panels . To demonstrate the specificity of staining, the binding of PE-TC11-18H10 was blocked by the preincubation of the fixed/permeabilized, stimulated EL4 cells with unlabeled TC11-18H10 antibody 10 mug; Cat. No. 559501, right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the unstimulated stained left panel and unlabeled antibody blocking right panel controls.