BD PharmingenTM PE Rat Anti-Mouse Ly-49A

Description

Flow cytometric analysis of Ly-49A expression on Mouse splenocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD49b antibody Cat No. 560628 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Mouse Anti-Rat Ly-49A antibody Cat. No. 569156/569157; Right Plot at 0.06 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of Ly-49A or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Ly-49A expression on Mouse splenocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD49b antibody Cat No. 560628 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Mouse Anti-Rat Ly-49A antibody Cat. No. 569156/569157; Right Plot at 0.06 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of Ly-49A or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Ly-49A expression on Mouse splenocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD49b antibody Cat No. 560628 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Mouse Anti-Rat Ly-49A antibody Cat. No. 569156/569157; Right Plot at 0.06 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of Ly-49A or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Ly-49A expression on Mouse splenocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD49b antibody Cat No. 560628 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Mouse Anti-Rat Ly-49A antibody Cat. No. 569156/569157; Right Plot at 0.06 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of Ly-49A or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Ly-49A expression on Mouse splenocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD49b antibody Cat No. 560628 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Mouse Anti-Rat Ly-49A antibody Cat. No. 569156/569157; Right Plot at 0.06 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of Ly-49A or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Ly-49A expression on Mouse splenocytes. BALB/c Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD49b antibody Cat No. 560628 and with either PE Rat IgG2b, kappa Isotype Control Cat. No. 555848; Left Plot or PE Mouse Anti-Rat Ly-49A antibody Cat. No. 569156/569157; Right Plot at 0.06 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of Ly-49A or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.