BD PharmingenTM PE Rat Anti-Mouse MerTK (Mer)

Description

Two-color flow cytometric analysis of MerTK Mer expression on normal Mouse peritoneal cavity cells PEC . PEC from C57BL/6 Mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat. No. 565853 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse MerTK Mer antibody Cat. No. 570196/570197; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of MerTK Mer or Ig Isotype control staining versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative PEC. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of MerTK Mer expression on normal Mouse peritoneal cavity cells PEC . PEC from C57BL/6 Mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat. No. 565853 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse MerTK Mer antibody Cat. No. 570196/570197; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of MerTK Mer or Ig Isotype control staining versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative PEC. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of MerTK Mer expression on normal Mouse peritoneal cavity cells PEC . PEC from C57BL/6 Mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat. No. 565853 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse MerTK Mer antibody Cat. No. 570196/570197; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of MerTK Mer or Ig Isotype control staining versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative PEC. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of MerTK Mer expression on normal Mouse peritoneal cavity cells PEC . PEC from C57BL/6 Mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat. No. 565853 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse MerTK Mer antibody Cat. No. 570196/570197; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of MerTK Mer or Ig Isotype control staining versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative PEC. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of MerTK Mer expression on normal Mouse peritoneal cavity cells PEC . PEC from C57BL/6 Mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat. No. 565853 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse MerTK Mer antibody Cat. No. 570196/570197; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of MerTK Mer or Ig Isotype control staining versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative PEC. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of MerTK Mer expression on normal Mouse peritoneal cavity cells PEC . PEC from C57BL/6 Mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat. No. 565853 and with either PE Rat IgG1, kappa Isotype Control Cat. No. 553925; Left Plot or PE Rat Anti-Mouse MerTK Mer antibody Cat. No. 570196/570197; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of MerTK Mer or Ig Isotype control staining versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative PEC. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.