BD PharmingenTM PE Rat Anti-Mouse P2X7

Description

Multicolor flow cytometric analysis of P2X7 receptor expression on mouse cells. Left Panel: C57BL/6 mouse peritoneal cells were stained with BD Horizon BV421 Rat Anti-Mouse F4/80 Cat. No. 565411 and APC Rat Anti-Mouse CD117 Cat. No. 553356 antibodies, and either PE Rat IgG2b, kappa Isotype Control Cat. No. 556925; dashed line histogram or PE Rat Anti-Mouse P2X7 antibody Cat. No. 565345; solid line histogram . The fluorescence histogram showing P2X7 expression or Ig Isotype control staining was derived from F4/80- CD117+ gated events with the forward and side light-scatter characteristics of viable cells. Right Panel: Mouse splenocytes were stained with BD Horizon BV421 Rat Anti-Mouse CD45R/B220 Cat. No. 562922; Left Plots and FITC Rat Anti-Mouse CD3 Molecular Complex Cat. No. 555274/561798; Right Plots antibodies, and either PE Rat IgG2b, kappa Isotype Control or PE Rat Anti-Mouse P2X7 antibody as indicated. Two-color flow cytometric dot plots showing the correlated expression of CD45R/B220 or CD3 versus P2X7 or Ig Isotype control staining were derived from gated events with the forward and side light- scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of P2X7 receptor expression on mouse cells. Left Panel: C57BL/6 mouse peritoneal cells were stained with BD Horizon BV421 Rat Anti-Mouse F4/80 Cat. No. 565411 and APC Rat Anti-Mouse CD117 Cat. No. 553356 antibodies, and either PE Rat IgG2b, kappa Isotype Control Cat. No. 556925; dashed line histogram or PE Rat Anti-Mouse P2X7 antibody Cat. No. 565345; solid line histogram . The fluorescence histogram showing P2X7 expression or Ig Isotype control staining was derived from F4/80- CD117+ gated events with the forward and side light-scatter characteristics of viable cells. Right Panel: Mouse splenocytes were stained with BD Horizon BV421 Rat Anti-Mouse CD45R/B220 Cat. No. 562922; Left Plots and FITC Rat Anti-Mouse CD3 Molecular Complex Cat. No. 555274/561798; Right Plots antibodies, and either PE Rat IgG2b, kappa Isotype Control or PE Rat Anti-Mouse P2X7 antibody as indicated. Two-color flow cytometric dot plots showing the correlated expression of CD45R/B220 or CD3 versus P2X7 or Ig Isotype control staining were derived from gated events with the forward and side light- scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of P2X7 receptor expression on mouse cells. Left Panel: C57BL/6 mouse peritoneal cells were stained with BD Horizon BV421 Rat Anti-Mouse F4/80 Cat. No. 565411 and APC Rat Anti-Mouse CD117 Cat. No. 553356 antibodies, and either PE Rat IgG2b, kappa Isotype Control Cat. No. 556925; dashed line histogram or PE Rat Anti-Mouse P2X7 antibody Cat. No. 565345; solid line histogram . The fluorescence histogram showing P2X7 expression or Ig Isotype control staining was derived from F4/80- CD117+ gated events with the forward and side light-scatter characteristics of viable cells. Right Panel: Mouse splenocytes were stained with BD Horizon BV421 Rat Anti-Mouse CD45R/B220 Cat. No. 562922; Left Plots and FITC Rat Anti-Mouse CD3 Molecular Complex Cat. No. 555274/561798; Right Plots antibodies, and either PE Rat IgG2b, kappa Isotype Control or PE Rat Anti-Mouse P2X7 antibody as indicated. Two-color flow cytometric dot plots showing the correlated expression of CD45R/B220 or CD3 versus P2X7 or Ig Isotype control staining were derived from gated events with the forward and side light- scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.