BD PharmingenTM PE Rat Anti-Mouse PDC-TREM

Description

Multi-color flow cytometric analysis of PDC-TREM expression on mouse plasmacytoid dentritic cells pDCs . Splenocytes from BALB/c mice were cultured in the presence of CpG-A Invivogen for 24 hours. The cells were then washed, and Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 or 553142 was added. The cells were stained simultaneously with optimal concentrations of FITC Hamster Anti-Mouse CD3e Cat. No. 553061, 553062, or 561827 , FITC Rat anti-Mouse CD19 Cat. No. 553785, 557398, or 561740 , APC Rat Anti-Mouse CD45R/B220 Cat. No. 553092 or 561880 , and BD Horizon BUV395 Hamster Anti-Mouse CD11c Cat. No. 564080 , plus either PE Rat IgG2a, kappa Isotype Control Cat.No. 553930; dashed line histogram or PE Rat Anti-Mouse PDC-TREM Cat. No. 566926; solid line histogram at 0.5 mug/test. The left plot was derived from CD3e-negative, CD19-negative viable leukocytes and displays the gating of the CD45R/B220-positive, CD11c-positive pDC population. The right plot shows the expression of PDC-TREM or Ig Isotype Control staining on the gated pDCs. Flow cytometry and data analysis was performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multi-color flow cytometric analysis of PDC-TREM expression on mouse plasmacytoid dentritic cells pDCs . Splenocytes from BALB/c mice were cultured in the presence of CpG-A Invivogen for 24 hours. The cells were then washed, and Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 or 553142 was added. The cells were stained simultaneously with optimal concentrations of FITC Hamster Anti-Mouse CD3e Cat. No. 553061, 553062, or 561827 , FITC Rat anti-Mouse CD19 Cat. No. 553785, 557398, or 561740 , APC Rat Anti-Mouse CD45R/B220 Cat. No. 553092 or 561880 , and BD Horizon BUV395 Hamster Anti-Mouse CD11c Cat. No. 564080 , plus either PE Rat IgG2a, kappa Isotype Control Cat.No. 553930; dashed line histogram or PE Rat Anti-Mouse PDC-TREM Cat. No. 566926; solid line histogram at 0.5 mug/test. The left plot was derived from CD3e-negative, CD19-negative viable leukocytes and displays the gating of the CD45R/B220-positive, CD11c-positive pDC population. The right plot shows the expression of PDC-TREM or Ig Isotype Control staining on the gated pDCs. Flow cytometry and data analysis was performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multi-color flow cytometric analysis of PDC-TREM expression on mouse plasmacytoid dentritic cells pDCs . Splenocytes from BALB/c mice were cultured in the presence of CpG-A Invivogen for 24 hours. The cells were then washed, and Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 or 553142 was added. The cells were stained simultaneously with optimal concentrations of FITC Hamster Anti-Mouse CD3e Cat. No. 553061, 553062, or 561827 , FITC Rat anti-Mouse CD19 Cat. No. 553785, 557398, or 561740 , APC Rat Anti-Mouse CD45R/B220 Cat. No. 553092 or 561880 , and BD Horizon BUV395 Hamster Anti-Mouse CD11c Cat. No. 564080 , plus either PE Rat IgG2a, kappa Isotype Control Cat.No. 553930; dashed line histogram or PE Rat Anti-Mouse PDC-TREM Cat. No. 566926; solid line histogram at 0.5 mug/test. The left plot was derived from CD3e-negative, CD19-negative viable leukocytes and displays the gating of the CD45R/B220-positive, CD11c-positive pDC population. The right plot shows the expression of PDC-TREM or Ig Isotype Control staining on the gated pDCs. Flow cytometry and data analysis was performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multi-color flow cytometric analysis of PDC-TREM expression on mouse plasmacytoid dentritic cells pDCs . Splenocytes from BALB/c mice were cultured in the presence of CpG-A Invivogen for 24 hours. The cells were then washed, and Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 or 553142 was added. The cells were stained simultaneously with optimal concentrations of FITC Hamster Anti-Mouse CD3e Cat. No. 553061, 553062, or 561827 , FITC Rat anti-Mouse CD19 Cat. No. 553785, 557398, or 561740 , APC Rat Anti-Mouse CD45R/B220 Cat. No. 553092 or 561880 , and BD Horizon BUV395 Hamster Anti-Mouse CD11c Cat. No. 564080 , plus either PE Rat IgG2a, kappa Isotype Control Cat.No. 553930; dashed line histogram or PE Rat Anti-Mouse PDC-TREM Cat. No. 566926; solid line histogram at 0.5 mug/test. The left plot was derived from CD3e-negative, CD19-negative viable leukocytes and displays the gating of the CD45R/B220-positive, CD11c-positive pDC population. The right plot shows the expression of PDC-TREM or Ig Isotype Control staining on the gated pDCs. Flow cytometry and data analysis was performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multi-color flow cytometric analysis of PDC-TREM expression on mouse plasmacytoid dentritic cells pDCs . Splenocytes from BALB/c mice were cultured in the presence of CpG-A Invivogen for 24 hours. The cells were then washed, and Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 or 553142 was added. The cells were stained simultaneously with optimal concentrations of FITC Hamster Anti-Mouse CD3e Cat. No. 553061, 553062, or 561827 , FITC Rat anti-Mouse CD19 Cat. No. 553785, 557398, or 561740 , APC Rat Anti-Mouse CD45R/B220 Cat. No. 553092 or 561880 , and BD Horizon BUV395 Hamster Anti-Mouse CD11c Cat. No. 564080 , plus either PE Rat IgG2a, kappa Isotype Control Cat.No. 553930; dashed line histogram or PE Rat Anti-Mouse PDC-TREM Cat. No. 566926; solid line histogram at 0.5 mug/test. The left plot was derived from CD3e-negative, CD19-negative viable leukocytes and displays the gating of the CD45R/B220-positive, CD11c-positive pDC population. The right plot shows the expression of PDC-TREM or Ig Isotype Control staining on the gated pDCs. Flow cytometry and data analysis was performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multi-color flow cytometric analysis of PDC-TREM expression on mouse plasmacytoid dentritic cells pDCs . Splenocytes from BALB/c mice were cultured in the presence of CpG-A Invivogen for 24 hours. The cells were then washed, and Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 or 553142 was added. The cells were stained simultaneously with optimal concentrations of FITC Hamster Anti-Mouse CD3e Cat. No. 553061, 553062, or 561827 , FITC Rat anti-Mouse CD19 Cat. No. 553785, 557398, or 561740 , APC Rat Anti-Mouse CD45R/B220 Cat. No. 553092 or 561880 , and BD Horizon BUV395 Hamster Anti-Mouse CD11c Cat. No. 564080 , plus either PE Rat IgG2a, kappa Isotype Control Cat.No. 553930; dashed line histogram or PE Rat Anti-Mouse PDC-TREM Cat. No. 566926; solid line histogram at 0.5 mug/test. The left plot was derived from CD3e-negative, CD19-negative viable leukocytes and displays the gating of the CD45R/B220-positive, CD11c-positive pDC population. The right plot shows the expression of PDC-TREM or Ig Isotype Control staining on the gated pDCs. Flow cytometry and data analysis was performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.