BD PharmingenTM PE Rat Anti-Mouse Siglec-F

Description

Two-color analysis of Siglec-F expression on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were simultaneously stained with FITC-conjugated anti-mouse CD11b Integrin alphaM chain mAb M1/70 Cat. No. 557396/553310 and either PE-conjugated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 553930, left panel or PE-conjugated mAb E50-2440 right panel in the presence of Mouse Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142 . Flow cytometry was performed on a FACSCalibur BD Biosciences, San Jose, CA . Two-color analysis of Siglec-F expression on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were simultaneously stained with FITC-conjugated anti-mouse CD11b Integrin alphaM chain mAb M1/70 Cat. No. 557396/553310 and either PE-conjugated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 553930, left panel or PE-conjugated mAb E50-2440 right panel in the presence of Mouse Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142 . Flow cytometry was performed on a FACSCalibur BD Biosciences, San Jose, CA . Two-color analysis of Siglec-F expression on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were simultaneously stained with FITC-conjugated anti-mouse CD11b Integrin alphaM chain mAb M1/70 Cat. No. 557396/553310 and either PE-conjugated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 553930, left panel or PE-conjugated mAb E50-2440 right panel in the presence of Mouse Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142 . Flow cytometry was performed on a FACSCalibur BD Biosciences, San Jose, CA . Show More Two-color analysis of Siglec-F expression on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were simultaneously stained with FITC-conjugated anti-mouse CD11b Integrin alphaM chain mAb M1/70 Cat. No. 557396/553310 and either PE-conjugated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 553930, left panel or PE-conjugated mAb E50-2440 right panel in the presence of Mouse Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142 . Flow cytometry was performed on a FACSCalibur BD Biosciences, San Jose, CA . Show More Two-color analysis of Siglec-F expression on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were simultaneously stained with FITC-conjugated anti-mouse CD11b Integrin alphaM chain mAb M1/70 Cat. No. 557396/553310 and either PE-conjugated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 553930, left panel or PE-conjugated mAb E50-2440 right panel in the presence of Mouse Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142 . Flow cytometry was performed on a FACSCalibur BD Biosciences, San Jose, CA . Two-color analysis of Siglec-F expression on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were simultaneously stained with FITC-conjugated anti-mouse CD11b Integrin alphaM chain mAb M1/70 Cat. No. 557396/553310 and either PE-conjugated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 553930, left panel or PE-conjugated mAb E50-2440 right panel in the presence of Mouse Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142 . Flow cytometry was performed on a FACSCalibur BD Biosciences, San Jose, CA .