BD PharmingenTM PE Rat Anti-Mouse TNF

Description

Expression of TNF by MiCK-1 cells. MiCK-1 cells Cat. No. 554652 were stained with 0.06 ug of FITC-conjugated rat anti-mouse CD4 FITC-RM4-5, Cat. No. 553047 , fixed, permeabilized, and subsequently stained with 0.06 ug of PE-conjugated rat anti-mouse TNF antibody PE-MP6-XT22, Cat. No. 554419 by using the Pharmingen staining protocol left panel . To demonstrate specificity of staining, the binding of PE-MP6-XT22 was blocked by the preincubation of the conjugated antibody with molar excess of recombinant mouse TNF 0.25 mug, Cat. No. 554589; right panel , and by preincubation of the fixed/permeabilized cells with an excess of the unlabelled MP6-XT22 mAb 2 mug, Cat. No. 554416; data not shown . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking right panel and unlabelled antibody blocking specificity controls. Expression of TNF by MiCK-1 cells. MiCK-1 cells Cat. No. 554652 were stained with 0.06 ug of FITC-conjugated rat anti-mouse CD4 FITC-RM4-5, Cat. No. 553047 , fixed, permeabilized, and subsequently stained with 0.06 ug of PE-conjugated rat anti-mouse TNF antibody PE-MP6-XT22, Cat. No. 554419 by using the Pharmingen staining protocol left panel . To demonstrate specificity of staining, the binding of PE-MP6-XT22 was blocked by the preincubation of the conjugated antibody with molar excess of recombinant mouse TNF 0.25 mug, Cat. No. 554589; right panel , and by preincubation of the fixed/permeabilized cells with an excess of the unlabelled MP6-XT22 mAb 2 mug, Cat. No. 554416; data not shown . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking right panel and unlabelled antibody blocking specificity controls. Expression of TNF by MiCK-1 cells. MiCK-1 cells Cat. No. 554652 were stained with 0.06 ug of FITC-conjugated rat anti-mouse CD4 FITC-RM4-5, Cat. No. 553047 , fixed, permeabilized, and subsequently stained with 0.06 ug of PE-conjugated rat anti-mouse TNF antibody PE-MP6-XT22, Cat. No. 554419 by using the Pharmingen staining protocol left panel . To demonstrate specificity of staining, the binding of PE-MP6-XT22 was blocked by the preincubation of the conjugated antibody with molar excess of recombinant mouse TNF 0.25 mug, Cat. No. 554589; right panel , and by preincubation of the fixed/permeabilized cells with an excess of the unlabelled MP6-XT22 mAb 2 mug, Cat. No. 554416; data not shown . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking right panel and unlabelled antibody blocking specificity controls. Show More Expression of TNF by MiCK-1 cells. MiCK-1 cells Cat. No. 554652 were stained with 0.06 ug of FITC-conjugated rat anti-mouse CD4 FITC-RM4-5, Cat. No. 553047 , fixed, permeabilized, and subsequently stained with 0.06 ug of PE-conjugated rat anti-mouse TNF antibody PE-MP6-XT22, Cat. No. 554419 by using the Pharmingen staining protocol left panel . To demonstrate specificity of staining, the binding of PE-MP6-XT22 was blocked by the preincubation of the conjugated antibody with molar excess of recombinant mouse TNF 0.25 mug, Cat. No. 554589; right panel , and by preincubation of the fixed/permeabilized cells with an excess of the unlabelled MP6-XT22 mAb 2 mug, Cat. No. 554416; data not shown . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking right panel and unlabelled antibody blocking specificity controls. Show More Expression of TNF by MiCK-1 cells. MiCK-1 cells Cat. No. 554652 were stained with 0.06 ug of FITC-conjugated rat anti-mouse CD4 FITC-RM4-5, Cat. No. 553047 , fixed, permeabilized, and subsequently stained with 0.06 ug of PE-conjugated rat anti-mouse TNF antibody PE-MP6-XT22, Cat. No. 554419 by using the Pharmingen staining protocol left panel . To demonstrate specificity of staining, the binding of PE-MP6-XT22 was blocked by the preincubation of the conjugated antibody with molar excess of recombinant mouse TNF 0.25 mug, Cat. No. 554589; right panel , and by preincubation of the fixed/permeabilized cells with an excess of the unlabelled MP6-XT22 mAb 2 mug, Cat. No. 554416; data not shown . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking right panel and unlabelled antibody blocking specificity controls. Expression of TNF by MiCK-1 cells. MiCK-1 cells Cat. No. 554652 were stained with 0.06 ug of FITC-conjugated rat anti-mouse CD4 FITC-RM4-5, Cat. No. 553047 , fixed, permeabilized, and subsequently stained with 0.06 ug of PE-conjugated rat anti-mouse TNF antibody PE-MP6-XT22, Cat. No. 554419 by using the Pharmingen staining protocol left panel . To demonstrate specificity of staining, the binding of PE-MP6-XT22 was blocked by the preincubation of the conjugated antibody with molar excess of recombinant mouse TNF 0.25 mug, Cat. No. 554589; right panel , and by preincubation of the fixed/permeabilized cells with an excess of the unlabelled MP6-XT22 mAb 2 mug, Cat. No. 554416; data not shown . The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking right panel and unlabelled antibody blocking specificity controls.