BD PharmingenTM PE Rat Anti-Mouse Zbtb46

Description

Analysis of Zbtb46 Expression Left Panel - Two-color flow cytometric analysis Zbtb46 expression in mouse splenocytes. Mouse splenocytes were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were stained with BD Horizon BV421 Armenian Hamster Anti-Mouse CD11c Cat. No. 565452 and either PE Rat IgG1, kappa Isotype Control Cat. No. 554685; Left Plot or PE Rat Anti-Mouse Zbtb46 antibody Cat. No. 565832; Right Plot . Two-color flow cytometric contour plots showing the correlated expression of Zbtb46 or Ig Isotype control staining versus CD11c were derived from gated events with the forward and side light-scatter characteristics of intact splenocytes. Right Panel. Multicolor flow cytometric analysis of Zbtb46 expression in mouse splenic conventional dendritic cells. Similarly fixed and permeabilized splenocytes were stained with BD Horizon BV421 Armenian Hamster Anti-Mouse CD11c, Alexa Fluor 647 Rat Anti-Mouse I-A/I-E Cat. No. 562367 , and either PE Rat IgG1, kappa Isotype Control dashed line histogram or PE Rat Anti-Mouse Zbtb46 antibody solid line histogram . The fluorescence histogram showing Zbtb46 expression or Ig Isotype control staining for conventional dendritic cells cDC was derived from CD11c-hi I-A/I-E-hi gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Analysis of Zbtb46 Expression Left Panel - Two-color flow cytometric analysis Zbtb46 expression in mouse splenocytes. Mouse splenocytes were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were stained with BD Horizon BV421 Armenian Hamster Anti-Mouse CD11c Cat. No. 565452 and either PE Rat IgG1, kappa Isotype Control Cat. No. 554685; Left Plot or PE Rat Anti-Mouse Zbtb46 antibody Cat. No. 565832; Right Plot . Two-color flow cytometric contour plots showing the correlated expression of Zbtb46 or Ig Isotype control staining versus CD11c were derived from gated events with the forward and side light-scatter characteristics of intact splenocytes. Right Panel. Multicolor flow cytometric analysis of Zbtb46 expression in mouse splenic conventional dendritic cells. Similarly fixed and permeabilized splenocytes were stained with BD Horizon BV421 Armenian Hamster Anti-Mouse CD11c, Alexa Fluor 647 Rat Anti-Mouse I-A/I-E Cat. No. 562367 , and either PE Rat IgG1, kappa Isotype Control dashed line histogram or PE Rat Anti-Mouse Zbtb46 antibody solid line histogram . The fluorescence histogram showing Zbtb46 expression or Ig Isotype control staining for conventional dendritic cells cDC was derived from CD11c-hi I-A/I-E-hi gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Show More Analysis of Zbtb46 Expression Left Panel - Two-color flow cytometric analysis Zbtb46 expression in mouse splenocytes. Mouse splenocytes were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were stained with BD Horizon BV421 Armenian Hamster Anti-Mouse CD11c Cat. No. 565452 and either PE Rat IgG1, kappa Isotype Control Cat. No. 554685; Left Plot or PE Rat Anti-Mouse Zbtb46 antibody Cat. No. 565832; Right Plot . Two-color flow cytometric contour plots showing the correlated expression of Zbtb46 or Ig Isotype control staining versus CD11c were derived from gated events with the forward and side light-scatter characteristics of intact splenocytes. Right Panel. Multicolor flow cytometric analysis of Zbtb46 expression in mouse splenic conventional dendritic cells. Similarly fixed and permeabilized splenocytes were stained with BD Horizon BV421 Armenian Hamster Anti-Mouse CD11c, Alexa Fluor 647 Rat Anti-Mouse I-A/I-E Cat. No. 562367 , and either PE Rat IgG1, kappa Isotype Control dashed line histogram or PE Rat Anti-Mouse Zbtb46 antibody solid line histogram . The fluorescence histogram showing Zbtb46 expression or Ig Isotype control staining for conventional dendritic cells cDC was derived from CD11c-hi I-A/I-E-hi gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System.