BD PharmingenTM PE Rat anti-Pax-5

Description

Multicolor flow cytometric analysis of Pax5 expression in human and mouse lymphocytes. Human peripheral blood mononuclear cells PBMC and BALB/c mouse splenocytes were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 and then stained with either PE Rat Anti-Mouse Pax5 antibody Cat. No. 562688 or PE rat IgG2a, kappa Isotype Control Cat. No. 554689 . The PBMC were counterstained with BD Horizon V450 Mouse Anti-Human CD3 Cat. No. 560366 and APC Mouse Anti-Human CD21 Cat. No. 559867 antibodies. Mouse splenocytes were counterstained with PerCP-Cy5.5 Rat Anti-Mouse CD45R/B220 Cat. No. 552771 and FITC Hamster Anti-Mouse CD3e Cat. No. 553062 antibodies. Upper Panel: Human Peripheral Blood Lymphocytes PBL : Contour plots from left to right showing the expression of CD21 versus Rat IgG2a kappa Isotype Control, CD21 versus Pax5 and CD3 versus Pax5, respectively. Lower Panel: Mouse Splenic Lymphocytes: Contour plots from left to right showing the expression of CD45R/B220 versus Rat IgG2a kappa Isotype Control, CD45R/B220 versus PE Pax5 and CD3e versus Pax5, respectively. The flow cytometric data were derived from gated events with the forward and side light-scatter characteristics of intact human top plots and mouse bottom plots lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of Pax5 expression in human and mouse lymphocytes. Human peripheral blood mononuclear cells PBMC and BALB/c mouse splenocytes were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 and then stained with either PE Rat Anti-Mouse Pax5 antibody Cat. No. 562688 or PE rat IgG2a, kappa Isotype Control Cat. No. 554689 . The PBMC were counterstained with BD Horizon V450 Mouse Anti-Human CD3 Cat. No. 560366 and APC Mouse Anti-Human CD21 Cat. No. 559867 antibodies. Mouse splenocytes were counterstained with PerCP-Cy5.5 Rat Anti-Mouse CD45R/B220 Cat. No. 552771 and FITC Hamster Anti-Mouse CD3e Cat. No. 553062 antibodies. Upper Panel: Human Peripheral Blood Lymphocytes PBL : Contour plots from left to right showing the expression of CD21 versus Rat IgG2a kappa Isotype Control, CD21 versus Pax5 and CD3 versus Pax5, respectively. Lower Panel: Mouse Splenic Lymphocytes: Contour plots from left to right showing the expression of CD45R/B220 versus Rat IgG2a kappa Isotype Control, CD45R/B220 versus PE Pax5 and CD3e versus Pax5, respectively. The flow cytometric data were derived from gated events with the forward and side light-scatter characteristics of intact human top plots and mouse bottom plots lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of Pax5 expression in human and mouse lymphocytes. Human peripheral blood mononuclear cells PBMC and BALB/c mouse splenocytes were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 and then stained with either PE Rat Anti-Mouse Pax5 antibody Cat. No. 562688 or PE rat IgG2a, kappa Isotype Control Cat. No. 554689 . The PBMC were counterstained with BD Horizon V450 Mouse Anti-Human CD3 Cat. No. 560366 and APC Mouse Anti-Human CD21 Cat. No. 559867 antibodies. Mouse splenocytes were counterstained with PerCP-Cy5.5 Rat Anti-Mouse CD45R/B220 Cat. No. 552771 and FITC Hamster Anti-Mouse CD3e Cat. No. 553062 antibodies. Upper Panel: Human Peripheral Blood Lymphocytes PBL : Contour plots from left to right showing the expression of CD21 versus Rat IgG2a kappa Isotype Control, CD21 versus Pax5 and CD3 versus Pax5, respectively. Lower Panel: Mouse Splenic Lymphocytes: Contour plots from left to right showing the expression of CD45R/B220 versus Rat IgG2a kappa Isotype Control, CD45R/B220 versus PE Pax5 and CD3e versus Pax5, respectively. The flow cytometric data were derived from gated events with the forward and side light-scatter characteristics of intact human top plots and mouse bottom plots lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.