BD PharmingenTM PE Rat Anti-SAP

Description

Flow cytometric analyses of SAP expression Panel 1. Spleen cells from a C57BL/6 mouse were fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash Buffer Cat. No. 554723 . The cells were stained with APC Hamster Anti-Mouse CD3e Cat. No. 553066 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-SAP antibody Cat. No. 566729; Right Plot at 0.125 mug/test. Two-color flow cytometric pseudocolor plots showing the correlated expression of SAP or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Panel 2. Human peripheral blood mononuclear cells PBMCs were similarly fixed and permeabilized and then stained with APC Mouse Anti-Human CD3 Cat. No. 561811 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-SAP antibody Right Plot at 0.125 mug/test. Pseudocolor dot plots showing the correlated expression of SAP or Ig Isotype control staining versus CD3 were derived from gated events with the light-scatter characteristics of intact lymphocytes. Panel 3. Cells from the human Jurkat Acute T Cell Leukemia, ATCC TIB-152 cell line were similarly fixed and permeabilized and then stained with PE Rat IgG2a, kappa Isotype Control dashed line histogram or PE Rat Anti-SAP antibody solid line histogram at 0.125 mug/test. The fluorescence histogram showing SAP expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analyses of SAP expression Panel 1. Spleen cells from a C57BL/6 mouse were fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash Buffer Cat. No. 554723 . The cells were stained with APC Hamster Anti-Mouse CD3e Cat. No. 553066 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-SAP antibody Cat. No. 566729; Right Plot at 0.125 mug/test. Two-color flow cytometric pseudocolor plots showing the correlated expression of SAP or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Panel 2. Human peripheral blood mononuclear cells PBMCs were similarly fixed and permeabilized and then stained with APC Mouse Anti-Human CD3 Cat. No. 561811 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-SAP antibody Right Plot at 0.125 mug/test. Pseudocolor dot plots showing the correlated expression of SAP or Ig Isotype control staining versus CD3 were derived from gated events with the light-scatter characteristics of intact lymphocytes. Panel 3. Cells from the human Jurkat Acute T Cell Leukemia, ATCC TIB-152 cell line were similarly fixed and permeabilized and then stained with PE Rat IgG2a, kappa Isotype Control dashed line histogram or PE Rat Anti-SAP antibody solid line histogram at 0.125 mug/test. The fluorescence histogram showing SAP expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analyses of SAP expression Panel 1. Spleen cells from a C57BL/6 mouse were fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash Buffer Cat. No. 554723 . The cells were stained with APC Hamster Anti-Mouse CD3e Cat. No. 553066 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-SAP antibody Cat. No. 566729; Right Plot at 0.125 mug/test. Two-color flow cytometric pseudocolor plots showing the correlated expression of SAP or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Panel 2. Human peripheral blood mononuclear cells PBMCs were similarly fixed and permeabilized and then stained with APC Mouse Anti-Human CD3 Cat. No. 561811 and either PE Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or PE Rat Anti-SAP antibody Right Plot at 0.125 mug/test. Pseudocolor dot plots showing the correlated expression of SAP or Ig Isotype control staining versus CD3 were derived from gated events with the light-scatter characteristics of intact lymphocytes. Panel 3. Cells from the human Jurkat Acute T Cell Leukemia, ATCC TIB-152 cell line were similarly fixed and permeabilized and then stained with PE Rat IgG2a, kappa Isotype Control dashed line histogram or PE Rat Anti-SAP antibody solid line histogram at 0.125 mug/test. The fluorescence histogram showing SAP expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.