
567181 ·
BDB Bioscience · Cat: 567181
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Description
Multiparameter flow cytometric analysis of CD27 expression on human peripheral blood leucocyte populations. Human whole blood was stained with either PerCP-Cy5.5 Mouse IgG1, kappa Isotype Control Cat. No. 550795; Left Plot or PerCP-Cy5.5 Mouse Anti-Human CD27 antibody Cat. No. 567181/567182; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD27 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD27 expression on human peripheral blood leucocyte populations. Human whole blood was stained with either PerCP-Cy5.5 Mouse IgG1, kappa Isotype Control Cat. No. 550795; Left Plot or PerCP-Cy5.5 Mouse Anti-Human CD27 antibody Cat. No. 567181/567182; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD27 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD27 expression on human peripheral blood leucocyte populations. Human whole blood was stained with either PerCP-Cy5.5 Mouse IgG1, kappa Isotype Control Cat. No. 550795; Left Plot or PerCP-Cy5.5 Mouse Anti-Human CD27 antibody Cat. No. 567181/567182; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . A bivariate pseudocolor density plot showing the correlated expression of CD27 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.