BD PharmingenTM PerCP-Cy5.5 Rat Anti-Mouse CD39

Description

Flow cytometric analysis of CD39 expression on Mouse splenic lymphocytes. BALB/c mouse splenocytes were stained with BD Horizon BUV395 Rat Anti-Mouse CD4 Cat. No. 563790/565974 and APC Rat Anti-Mouse CD25 Cat. No. 557192/561048 antibodies, and with either PerCP-Cy5.5 Rat IgG2b, kappa Isotype Control Cat. No. 550764; Left Plots or PerCP-Cy5.5 Rat Anti-Mouse CD39 antibody Cat. No. 567270; Right Plots at 0.125 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of CD39 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Lower Plots: Bivariate pseudocolor density plots showing the correlated expression CD39 or Ig Isotype control staining versus CD25 were derived from CD4-positive gated events with the light scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometric analysis of CD39 expression on Mouse splenic lymphocytes. BALB/c mouse splenocytes were stained with BD Horizon BUV395 Rat Anti-Mouse CD4 Cat. No. 563790/565974 and APC Rat Anti-Mouse CD25 Cat. No. 557192/561048 antibodies, and with either PerCP-Cy5.5 Rat IgG2b, kappa Isotype Control Cat. No. 550764; Left Plots or PerCP-Cy5.5 Rat Anti-Mouse CD39 antibody Cat. No. 567270; Right Plots at 0.125 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of CD39 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Lower Plots: Bivariate pseudocolor density plots showing the correlated expression CD39 or Ig Isotype control staining versus CD25 were derived from CD4-positive gated events with the light scatter characteristics of viable 7-AAD-negative lymphocytes. Show More Flow cytometric analysis of CD39 expression on Mouse splenic lymphocytes. BALB/c mouse splenocytes were stained with BD Horizon BUV395 Rat Anti-Mouse CD4 Cat. No. 563790/565974 and APC Rat Anti-Mouse CD25 Cat. No. 557192/561048 antibodies, and with either PerCP-Cy5.5 Rat IgG2b, kappa Isotype Control Cat. No. 550764; Left Plots or PerCP-Cy5.5 Rat Anti-Mouse CD39 antibody Cat. No. 567270; Right Plots at 0.125 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of CD39 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Lower Plots: Bivariate pseudocolor density plots showing the correlated expression CD39 or Ig Isotype control staining versus CD25 were derived from CD4-positive gated events with the light scatter characteristics of viable 7-AAD-negative lymphocytes.