BD PharmingenTM PerCP-Cy5.5 Rat Anti-Mouse CD8b

Description

Multicolor flow cytometric analysis of CD8b expression on mouse splenocytes. AKR/J mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . Cells were stained with APC Hamster Anti-Mouse CD3e antbody Cat. No. 553066/561826 and either PerCP-Cy5.5 Rat IgG2b, kappa Isotype Control Cat. No. 550764; Left Plot or PerCP-Cy5.5 Rat Anti-Mouse CD8b antibody Cat. No. 567597; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing correlated CD8b expression or Ig Isotype control staining versus CD3e was derived from gated events with the side and forward light-scattering characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD8b expression on mouse splenocytes. AKR/J mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . Cells were stained with APC Hamster Anti-Mouse CD3e antbody Cat. No. 553066/561826 and either PerCP-Cy5.5 Rat IgG2b, kappa Isotype Control Cat. No. 550764; Left Plot or PerCP-Cy5.5 Rat Anti-Mouse CD8b antibody Cat. No. 567597; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing correlated CD8b expression or Ig Isotype control staining versus CD3e was derived from gated events with the side and forward light-scattering characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD8b expression on mouse splenocytes. AKR/J mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . Cells were stained with APC Hamster Anti-Mouse CD3e antbody Cat. No. 553066/561826 and either PerCP-Cy5.5 Rat IgG2b, kappa Isotype Control Cat. No. 550764; Left Plot or PerCP-Cy5.5 Rat Anti-Mouse CD8b antibody Cat. No. 567597; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing correlated CD8b expression or Ig Isotype control staining versus CD3e was derived from gated events with the side and forward light-scattering characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD8b expression on mouse splenocytes. AKR/J mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . Cells were stained with APC Hamster Anti-Mouse CD3e antbody Cat. No. 553066/561826 and either PerCP-Cy5.5 Rat IgG2b, kappa Isotype Control Cat. No. 550764; Left Plot or PerCP-Cy5.5 Rat Anti-Mouse CD8b antibody Cat. No. 567597; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing correlated CD8b expression or Ig Isotype control staining versus CD3e was derived from gated events with the side and forward light-scattering characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD8b expression on mouse splenocytes. AKR/J mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . Cells were stained with APC Hamster Anti-Mouse CD3e antbody Cat. No. 553066/561826 and either PerCP-Cy5.5 Rat IgG2b, kappa Isotype Control Cat. No. 550764; Left Plot or PerCP-Cy5.5 Rat Anti-Mouse CD8b antibody Cat. No. 567597; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing correlated CD8b expression or Ig Isotype control staining versus CD3e was derived from gated events with the side and forward light-scattering characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD8b expression on mouse splenocytes. AKR/J mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . Cells were stained with APC Hamster Anti-Mouse CD3e antbody Cat. No. 553066/561826 and either PerCP-Cy5.5 Rat IgG2b, kappa Isotype Control Cat. No. 550764; Left Plot or PerCP-Cy5.5 Rat Anti-Mouse CD8b antibody Cat. No. 567597; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing correlated CD8b expression or Ig Isotype control staining versus CD3e was derived from gated events with the side and forward light-scattering characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.