BD PharmingenTM PerCP-CyTM5.5 Mouse Anti-Bcl-6

Description

Flow cytometric analysis of Bcl-6 expression on Human Ramos Left Panel . Ramos cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , followed by intracellular staining with either PerCP-Cy5.5 Mouse anti-Human Bcl-6 antibody Cat. No. 562198, solid line histogram or a PerCP-Cy5.5 mIgG1, kappa isotype control Cat. No. 550795; dashed line histogram . Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Multicolor flow cytometric analysis of Bcl-6 expression on Mouse B lymphocytes Middle and Right Panel . BALB/c mesenteric lymph node cells were stained with APC Rat Anti-Mouse B220 Cat. No. 553092/561880 , Alexa Fluor 647 Rat Anti-Mouse CD4 Cat. No. 557956/561025 , FITC Hamster Anti-Mouse Fas/CD95 Cat. No. 554257 , and BD Horizon V450 Rat Anti-Mouse IgD Cat. No. 560869 . Cells were washed, resuspended in RPMI with 10 % FBS, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with PerCP-Cy5.5 Mouse Anti-Human Bcl-6 Cat. No. 562198 . The two-color flow cytometric dot plot shows the expression of IgD versus Fas/CD95 by B cells identified as CD4-B220+ from gated events with the forward and side light-scatter characteristics of intact lymphocytes Middle Panel . Germinal center B cells were identified as IgDloFas+ B lymphocytes. Flow cytometric fluorescence histograms Right Panel show intracellular Bcl-6 staining of mouse germinal center B cells solid line histogram and non-GC B cells dashed line histogram . Flow cytometry was performed using a BD LSR II Flow Cytometry System. Flow cytometric analysis of Bcl-6 expression on Human Ramos Left Panel . Ramos cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , followed by intracellular staining with either PerCP-Cy5.5 Mouse anti-Human Bcl-6 antibody Cat. No. 562198, solid line histogram or a PerCP-Cy5.5 mIgG1, kappa isotype control Cat. No. 550795; dashed line histogram . Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Show More Multicolor flow cytometric analysis of Bcl-6 expression on Mouse B lymphocytes Middle and Right Panel . BALB/c mesenteric lymph node cells were stained with APC Rat Anti-Mouse B220 Cat. No. 553092/561880 , Alexa Fluor 647 Rat Anti-Mouse CD4 Cat. No. 557956/561025 , FITC Hamster Anti-Mouse Fas/CD95 Cat. No. 554257 , and BD Horizon V450 Rat Anti-Mouse IgD Cat. No. 560869 . Cells were washed, resuspended in RPMI with 10 % FBS, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with PerCP-Cy5.5 Mouse Anti-Human Bcl-6 Cat. No. 562198 . The two-color flow cytometric dot plot shows the expression of IgD versus Fas/CD95 by B cells identified as CD4-B220+ from gated events with the forward and side light-scatter characteristics of intact lymphocytes Middle Panel . Germinal center B cells were identified as IgDloFas+ B lymphocytes. Flow cytometric fluorescence histograms Right Panel show intracellular Bcl-6 staining of mouse germinal center B cells solid line histogram and non-GC B cells dashed line histogram . Flow cytometry was performed using a BD LSR II Flow Cytometry System. Show More Flow cytometric analysis of Bcl-6 expression on Human Ramos Left Panel . Ramos cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , followed by intracellular staining with either PerCP-Cy5.5 Mouse anti-Human Bcl-6 antibody Cat. No. 562198, solid line histogram or a PerCP-Cy5.5 mIgG1, kappa isotype control Cat. No. 550795; dashed line histogram . Flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Multicolor flow cytometric analysis of Bcl-6 expression on Mouse B lymphocytes Middle and Right Panel . BALB/c mesenteric lymph node cells were stained with APC Rat Anti-Mouse B220 Cat. No. 553092/561880 , Alexa Fluor 647 Rat Anti-Mouse CD4 Cat. No. 557956/561025 , FITC Hamster Anti-Mouse Fas/CD95 Cat. No. 554257 , and BD Horizon V450 Rat Anti-Mouse IgD Cat. No. 560869 . Cells were washed, resuspended in RPMI with 10 % FBS, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with PerCP-Cy5.5 Mouse Anti-Human Bcl-6 Cat. No. 562198 . The two-color flow cytometric dot plot shows the expression of IgD versus Fas/CD95 by B cells identified as CD4-B220+ from gated events with the forward and side light-scatter characteristics of intact lymphocytes Middle Panel . Germinal center B cells were identified as IgDloFas+ B lymphocytes. Flow cytometric fluorescence histograms Right Panel show intracellular Bcl-6 staining of mouse germinal center B cells solid line histogram and non-GC B cells dashed line histogram . Flow cytometry was performed using a BD LSR II Flow Cytometry System.