BD PharmingenTM PerCP-CyTM5.5 Mouse Anti-Human IL-17A

Description

Flow cytometric analysis of IL-17A in stimulated human lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with PMA and Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/ Cytoperm reagents Cat. No. 554714 followed by staining with PerCP-Cy5.5 anti-human IL-17A, FITC anti-human IFN-gamma Cat. No. 554700 , and APC anti-human IL-4 Cat. No. 554486 . Two-color flow cytometric dot plots showing the correlated expression patterns of IL-17A vs IFN-gamma Left panel or IL-4 Right panel were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSRII System. Flow cytometric analysis of IL-17A in stimulated human lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with PMA and Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/ Cytoperm reagents Cat. No. 554714 followed by staining with PerCP-Cy5.5 anti-human IL-17A, FITC anti-human IFN-gamma Cat. No. 554700 , and APC anti-human IL-4 Cat. No. 554486 . Two-color flow cytometric dot plots showing the correlated expression patterns of IL-17A vs IFN-gamma Left panel or IL-4 Right panel were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSRII System. Flow cytometric analysis of IL-17A in stimulated human lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with PMA and Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/ Cytoperm reagents Cat. No. 554714 followed by staining with PerCP-Cy5.5 anti-human IL-17A, FITC anti-human IFN-gamma Cat. No. 554700 , and APC anti-human IL-4 Cat. No. 554486 . Two-color flow cytometric dot plots showing the correlated expression patterns of IL-17A vs IFN-gamma Left panel or IL-4 Right panel were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSRII System. Show More Flow cytometric analysis of IL-17A in stimulated human lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with PMA and Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/ Cytoperm reagents Cat. No. 554714 followed by staining with PerCP-Cy5.5 anti-human IL-17A, FITC anti-human IFN-gamma Cat. No. 554700 , and APC anti-human IL-4 Cat. No. 554486 . Two-color flow cytometric dot plots showing the correlated expression patterns of IL-17A vs IFN-gamma Left panel or IL-4 Right panel were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSRII System. Show More Flow cytometric analysis of IL-17A in stimulated human lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with PMA and Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/ Cytoperm reagents Cat. No. 554714 followed by staining with PerCP-Cy5.5 anti-human IL-17A, FITC anti-human IFN-gamma Cat. No. 554700 , and APC anti-human IL-4 Cat. No. 554486 . Two-color flow cytometric dot plots showing the correlated expression patterns of IL-17A vs IFN-gamma Left panel or IL-4 Right panel were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSRII System. Flow cytometric analysis of IL-17A in stimulated human lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with PMA and Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/ Cytoperm reagents Cat. No. 554714 followed by staining with PerCP-Cy5.5 anti-human IL-17A, FITC anti-human IFN-gamma Cat. No. 554700 , and APC anti-human IL-4 Cat. No. 554486 . Two-color flow cytometric dot plots showing the correlated expression patterns of IL-17A vs IFN-gamma Left panel or IL-4 Right panel were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSRII System.