BD PharmingenTM PerCP-CyTM5.5 Mouse Anti-Human LAP

Description

Multicolor flow cytometric analysis of LAP expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 24 h with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The cells were harvested and stained with FITC Mouse Anti-Human CD4 Cat. No. 340133 and either PerCP-Cy5.5 Mouse IgG1, kappa Isotype Control Cat. No. 550795; Left Panel or PerCP-Cy5.5 Mouse Anti-Human LAP Cat. No. 562544; Right Panel antibodies. The cells were then fixed and stained with the Alexa Fluor 647 Mouse Anti-Human FoxP3 Cat. No. 560889/560045 antibody according to the recommended protocol. Two-color flow cytometric dot plots showing the correlated expression patterns of FoxP3 versus LAP or Ig isotype control staining were derived from CD4 positive-gated cells with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Clone TW4-2F8 is routinely quality tested through intracellular staining of LAP in P3UI-TGFbeta1 transfected cells. Multicolor flow cytometric analysis of LAP expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 24 h with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The cells were harvested and stained with FITC Mouse Anti-Human CD4 Cat. No. 340133 and either PerCP-Cy5.5 Mouse IgG1, kappa Isotype Control Cat. No. 550795; Left Panel or PerCP-Cy5.5 Mouse Anti-Human LAP Cat. No. 562544; Right Panel antibodies. The cells were then fixed and stained with the Alexa Fluor 647 Mouse Anti-Human FoxP3 Cat. No. 560889/560045 antibody according to the recommended protocol. Two-color flow cytometric dot plots showing the correlated expression patterns of FoxP3 versus LAP or Ig isotype control staining were derived from CD4 positive-gated cells with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Clone TW4-2F8 is routinely quality tested through intracellular staining of LAP in P3UI-TGFbeta1 transfected cells. Multicolor flow cytometric analysis of LAP expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 24 h with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The cells were harvested and stained with FITC Mouse Anti-Human CD4 Cat. No. 340133 and either PerCP-Cy5.5 Mouse IgG1, kappa Isotype Control Cat. No. 550795; Left Panel or PerCP-Cy5.5 Mouse Anti-Human LAP Cat. No. 562544; Right Panel antibodies. The cells were then fixed and stained with the Alexa Fluor 647 Mouse Anti-Human FoxP3 Cat. No. 560889/560045 antibody according to the recommended protocol. Two-color flow cytometric dot plots showing the correlated expression patterns of FoxP3 versus LAP or Ig isotype control staining were derived from CD4 positive-gated cells with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Clone TW4-2F8 is routinely quality tested through intracellular staining of LAP in P3UI-TGFbeta1 transfected cells. Show More Multicolor flow cytometric analysis of LAP expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 24 h with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The cells were harvested and stained with FITC Mouse Anti-Human CD4 Cat. No. 340133 and either PerCP-Cy5.5 Mouse IgG1, kappa Isotype Control Cat. No. 550795; Left Panel or PerCP-Cy5.5 Mouse Anti-Human LAP Cat. No. 562544; Right Panel antibodies. The cells were then fixed and stained with the Alexa Fluor 647 Mouse Anti-Human FoxP3 Cat. No. 560889/560045 antibody according to the recommended protocol. Two-color flow cytometric dot plots showing the correlated expression patterns of FoxP3 versus LAP or Ig isotype control staining were derived from CD4 positive-gated cells with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Clone TW4-2F8 is routinely quality tested through intracellular staining of LAP in P3UI-TGFbeta1 transfected cells. Show More Multicolor flow cytometric analysis of LAP expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 24 h with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The cells were harvested and stained with FITC Mouse Anti-Human CD4 Cat. No. 340133 and either PerCP-Cy5.5 Mouse IgG1, kappa Isotype Control Cat. No. 550795; Left Panel or PerCP-Cy5.5 Mouse Anti-Human LAP Cat. No. 562544; Right Panel antibodies. The cells were then fixed and stained with the Alexa Fluor 647 Mouse Anti-Human FoxP3 Cat. No. 560889/560045 antibody according to the recommended protocol. Two-color flow cytometric dot plots showing the correlated expression patterns of FoxP3 versus LAP or Ig isotype control staining were derived from CD4 positive-gated cells with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Clone TW4-2F8 is routinely quality tested through intracellular staining of LAP in P3UI-TGFbeta1 transfected cells. Multicolor flow cytometric analysis of LAP expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were activated 24 h with plate-bound Purified NA/LE Mouse Anti-Human CD3 Cat. No. 555329 and Purified NA/LE Mouse Anti-Human CD28 Cat. No. 555725 antibodies. The cells were harvested and stained with FITC Mouse Anti-Human CD4 Cat. No. 340133 and either PerCP-Cy5.5 Mouse IgG1, kappa Isotype Control Cat. No. 550795; Left Panel or PerCP-Cy5.5 Mouse Anti-Human LAP Cat. No. 562544; Right Panel antibodies. The cells were then fixed and stained with the Alexa Fluor 647 Mouse Anti-Human FoxP3 Cat. No. 560889/560045 antibody according to the recommended protocol. Two-color flow cytometric dot plots showing the correlated expression patterns of FoxP3 versus LAP or Ig isotype control staining were derived from CD4 positive-gated cells with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Clone TW4-2F8 is routinely quality tested through intracellular staining of LAP in P3UI-TGFbeta1 transfected cells.