BD PharmingenTM PerCP-CyTM5.5 Mouse Anti-Human TNF

Description

Flow cytometric analysis for TNF in stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with 50 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 500 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 in the presence of BD GolgiStop Cat. No. 554724 . Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554714 followed by staining with either a PerCP-Cy5.5 Mouse IgG1, kappa isotype control Cat. No. 550795; left panel or with the PerCP-Cy5.5 Mouse Anti-Human TNF antibody Cat. No. 560679; right panel . Dot plots were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis for TNF in stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with 50 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 500 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 in the presence of BD GolgiStop Cat. No. 554724 . Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554714 followed by staining with either a PerCP-Cy5.5 Mouse IgG1, kappa isotype control Cat. No. 550795; left panel or with the PerCP-Cy5.5 Mouse Anti-Human TNF antibody Cat. No. 560679; right panel . Dot plots were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis for TNF in stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with 50 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 500 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 in the presence of BD GolgiStop Cat. No. 554724 . Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554714 followed by staining with either a PerCP-Cy5.5 Mouse IgG1, kappa isotype control Cat. No. 550795; left panel or with the PerCP-Cy5.5 Mouse Anti-Human TNF antibody Cat. No. 560679; right panel . Dot plots were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Flow cytometric analysis for TNF in stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with 50 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 500 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 in the presence of BD GolgiStop Cat. No. 554724 . Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554714 followed by staining with either a PerCP-Cy5.5 Mouse IgG1, kappa isotype control Cat. No. 550795; left panel or with the PerCP-Cy5.5 Mouse Anti-Human TNF antibody Cat. No. 560679; right panel . Dot plots were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Flow cytometric analysis for TNF in stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with 50 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 500 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 in the presence of BD GolgiStop Cat. No. 554724 . Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554714 followed by staining with either a PerCP-Cy5.5 Mouse IgG1, kappa isotype control Cat. No. 550795; left panel or with the PerCP-Cy5.5 Mouse Anti-Human TNF antibody Cat. No. 560679; right panel . Dot plots were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis for TNF in stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with 50 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 500 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 in the presence of BD GolgiStop Cat. No. 554724 . Cells were then fixed and permeabilized using BD Cytofix/Cytoperm Cat. No. 554714 followed by staining with either a PerCP-Cy5.5 Mouse IgG1, kappa isotype control Cat. No. 550795; left panel or with the PerCP-Cy5.5 Mouse Anti-Human TNF antibody Cat. No. 560679; right panel . Dot plots were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system.