BD PharmingenTM PerCP-CyTM5.5 Mouse anti-PDX-1

Description

Flow cytometric analysis of PDX-1 in mouse pancreatic tumor insulinoma cells. Beta-TC-6 ATCC CRL-11506 cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No.558050 . The cells were stained with either PerCP-Cy5.5 Mouse IgG1, isotype control Cat. No. 550795; dashed line histogram or PerCP-Cy5.5 Mouse Anti-PDX-1 monoclonal antibody Cat. No. 563436, solid line histogram at matched concentrations. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. This antibody has been found to be reactive with both mouse and human PDX-1, and it can also be used on fixed cells that were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 . Flow cytometric analysis of PDX-1 in mouse pancreatic tumor insulinoma cells. Beta-TC-6 ATCC CRL-11506 cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No.558050 . The cells were stained with either PerCP-Cy5.5 Mouse IgG1, isotype control Cat. No. 550795; dashed line histogram or PerCP-Cy5.5 Mouse Anti-PDX-1 monoclonal antibody Cat. No. 563436, solid line histogram at matched concentrations. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. This antibody has been found to be reactive with both mouse and human PDX-1, and it can also be used on fixed cells that were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 . Show More Flow cytometric analysis of PDX-1 in mouse pancreatic tumor insulinoma cells. Beta-TC-6 ATCC CRL-11506 cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No.558050 . The cells were stained with either PerCP-Cy5.5 Mouse IgG1, isotype control Cat. No. 550795; dashed line histogram or PerCP-Cy5.5 Mouse Anti-PDX-1 monoclonal antibody Cat. No. 563436, solid line histogram at matched concentrations. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. This antibody has been found to be reactive with both mouse and human PDX-1, and it can also be used on fixed cells that were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 .