BD PharmingenTM PerCP-CyTM5.5 Rat Anti-Mouse CD223

Description

Two-color flow cytometric analysis of CD223 expression on activated mouse splenocytes. Mouse splenic leucocytes were stimulated in culture for 3 days with immobilized Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 . The cells were harvested, pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with BD Horizon BUV737 Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 564380 and either PerCP-Cy5.5 Rat IgG1, kappa Isotype Control Cat. No. 560537; Left Panel or PerCP-Cy5.5 Rat Anti-Mouse CD223 antibody Cat. No. 564673; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of CD223 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable activated splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD223 expression on activated mouse splenocytes. Mouse splenic leucocytes were stimulated in culture for 3 days with immobilized Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 . The cells were harvested, pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with BD Horizon BUV737 Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 564380 and either PerCP-Cy5.5 Rat IgG1, kappa Isotype Control Cat. No. 560537; Left Panel or PerCP-Cy5.5 Rat Anti-Mouse CD223 antibody Cat. No. 564673; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of CD223 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable activated splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD223 expression on activated mouse splenocytes. Mouse splenic leucocytes were stimulated in culture for 3 days with immobilized Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 . The cells were harvested, pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with BD Horizon BUV737 Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 564380 and either PerCP-Cy5.5 Rat IgG1, kappa Isotype Control Cat. No. 560537; Left Panel or PerCP-Cy5.5 Rat Anti-Mouse CD223 antibody Cat. No. 564673; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of CD223 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable activated splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of CD223 expression on activated mouse splenocytes. Mouse splenic leucocytes were stimulated in culture for 3 days with immobilized Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 . The cells were harvested, pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with BD Horizon BUV737 Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 564380 and either PerCP-Cy5.5 Rat IgG1, kappa Isotype Control Cat. No. 560537; Left Panel or PerCP-Cy5.5 Rat Anti-Mouse CD223 antibody Cat. No. 564673; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of CD223 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable activated splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of CD223 expression on activated mouse splenocytes. Mouse splenic leucocytes were stimulated in culture for 3 days with immobilized Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 . The cells were harvested, pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with BD Horizon BUV737 Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 564380 and either PerCP-Cy5.5 Rat IgG1, kappa Isotype Control Cat. No. 560537; Left Panel or PerCP-Cy5.5 Rat Anti-Mouse CD223 antibody Cat. No. 564673; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of CD223 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable activated splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD223 expression on activated mouse splenocytes. Mouse splenic leucocytes were stimulated in culture for 3 days with immobilized Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 . The cells were harvested, pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and then stained with BD Horizon BUV737 Rat Anti-Mouse CD3 Molecular Complex antibody Cat. No. 564380 and either PerCP-Cy5.5 Rat IgG1, kappa Isotype Control Cat. No. 560537; Left Panel or PerCP-Cy5.5 Rat Anti-Mouse CD223 antibody Cat. No. 564673; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of CD223 or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable activated splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.