BD PharmingenTM PerCP-CyTM5.5 Rat Anti-Mouse CD25

Description

Flow cytometric analysis of CD25 expression on stimulated mouse splenocytes. Freshly prepared mouse splenic leucocytes were stimulated with concanavalin A for 3 days, then stained with either PerCP-Cy5.5 Rat IgG1, lambda Isotype Control Cat. No. 551072; dashed line histogram or PerCP-Cy5.5 Rat Anti-Mouse CD25 antibody Cat. No. 551071/ 561112; solid line histogram at 1 mug/test. Fluorescence histogram showing CD25 or Ig Isotype expression was derived from gated events with the forward and side light- scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD FACSCanto Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD25 expression on stimulated mouse splenocytes. Freshly prepared mouse splenic leucocytes were stimulated with concanavalin A for 3 days, then stained with either PerCP-Cy5.5 Rat IgG1, lambda Isotype Control Cat. No. 551072; dashed line histogram or PerCP-Cy5.5 Rat Anti-Mouse CD25 antibody Cat. No. 551071/ 561112; solid line histogram at 1 mug/test. Fluorescence histogram showing CD25 or Ig Isotype expression was derived from gated events with the forward and side light- scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD FACSCanto Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD25 expression on stimulated mouse splenocytes. Freshly prepared mouse splenic leucocytes were stimulated with concanavalin A for 3 days, then stained with either PerCP-Cy5.5 Rat IgG1, lambda Isotype Control Cat. No. 551072; dashed line histogram or PerCP-Cy5.5 Rat Anti-Mouse CD25 antibody Cat. No. 551071/ 561112; solid line histogram at 1 mug/test. Fluorescence histogram showing CD25 or Ig Isotype expression was derived from gated events with the forward and side light- scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD FACSCanto Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD25 expression on stimulated mouse splenocytes. Freshly prepared mouse splenic leucocytes were stimulated with concanavalin A for 3 days, then stained with either PerCP-Cy5.5 Rat IgG1, lambda Isotype Control Cat. No. 551072; dashed line histogram or PerCP-Cy5.5 Rat Anti-Mouse CD25 antibody Cat. No. 551071/ 561112; solid line histogram at 1 mug/test. Fluorescence histogram showing CD25 or Ig Isotype expression was derived from gated events with the forward and side light- scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD FACSCanto Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD25 expression on stimulated mouse splenocytes. Freshly prepared mouse splenic leucocytes were stimulated with concanavalin A for 3 days, then stained with either PerCP-Cy5.5 Rat IgG1, lambda Isotype Control Cat. No. 551072; dashed line histogram or PerCP-Cy5.5 Rat Anti-Mouse CD25 antibody Cat. No. 551071/ 561112; solid line histogram at 1 mug/test. Fluorescence histogram showing CD25 or Ig Isotype expression was derived from gated events with the forward and side light- scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD FACSCanto Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD25 expression on stimulated mouse splenocytes. Freshly prepared mouse splenic leucocytes were stimulated with concanavalin A for 3 days, then stained with either PerCP-Cy5.5 Rat IgG1, lambda Isotype Control Cat. No. 551072; dashed line histogram or PerCP-Cy5.5 Rat Anti-Mouse CD25 antibody Cat. No. 551071/ 561112; solid line histogram at 1 mug/test. Fluorescence histogram showing CD25 or Ig Isotype expression was derived from gated events with the forward and side light- scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD FACSCanto Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.