BD PharmingenTM PerCP-CyTM5.5 Rat Anti-Mouse Foxp3

Description

Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Mouse splenic leucocytes were fixed and permeabilized with the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and either PerCP-Cy5.5 Rat IgG2a, kappa Isotype Control Cat. No. 550765; Left Plot or PerCP-Cy5.5 Rat Anti-Mouse Foxp3 Cat. No. 563902; Right Plot antibodies at 0.5 mug/test. The bivariate pseudocolor density plot shows the correlated expression patterns of Foxp3 or Ig Isotype control staining versus CD4 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data was performed using a BD LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Mouse splenic leucocytes were fixed and permeabilized with the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and either PerCP-Cy5.5 Rat IgG2a, kappa Isotype Control Cat. No. 550765; Left Plot or PerCP-Cy5.5 Rat Anti-Mouse Foxp3 Cat. No. 563902; Right Plot antibodies at 0.5 mug/test. The bivariate pseudocolor density plot shows the correlated expression patterns of Foxp3 or Ig Isotype control staining versus CD4 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data was performed using a BD LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Mouse splenic leucocytes were fixed and permeabilized with the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and either PerCP-Cy5.5 Rat IgG2a, kappa Isotype Control Cat. No. 550765; Left Plot or PerCP-Cy5.5 Rat Anti-Mouse Foxp3 Cat. No. 563902; Right Plot antibodies at 0.5 mug/test. The bivariate pseudocolor density plot shows the correlated expression patterns of Foxp3 or Ig Isotype control staining versus CD4 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data was performed using a BD LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Mouse splenic leucocytes were fixed and permeabilized with the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and either PerCP-Cy5.5 Rat IgG2a, kappa Isotype Control Cat. No. 550765; Left Plot or PerCP-Cy5.5 Rat Anti-Mouse Foxp3 Cat. No. 563902; Right Plot antibodies at 0.5 mug/test. The bivariate pseudocolor density plot shows the correlated expression patterns of Foxp3 or Ig Isotype control staining versus CD4 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data was performed using a BD LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Mouse splenic leucocytes were fixed and permeabilized with the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and either PerCP-Cy5.5 Rat IgG2a, kappa Isotype Control Cat. No. 550765; Left Plot or PerCP-Cy5.5 Rat Anti-Mouse Foxp3 Cat. No. 563902; Right Plot antibodies at 0.5 mug/test. The bivariate pseudocolor density plot shows the correlated expression patterns of Foxp3 or Ig Isotype control staining versus CD4 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data was performed using a BD LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Mouse splenic leucocytes were fixed and permeabilized with the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047/553046/561835 and either PerCP-Cy5.5 Rat IgG2a, kappa Isotype Control Cat. No. 550765; Left Plot or PerCP-Cy5.5 Rat Anti-Mouse Foxp3 Cat. No. 563902; Right Plot antibodies at 0.5 mug/test. The bivariate pseudocolor density plot shows the correlated expression patterns of Foxp3 or Ig Isotype control staining versus CD4 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data was performed using a BD LSR II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.