BD PharmingenTM PerCP-CyTM5.5 Rat Anti-Mouse IgD

Description

Two-color flow cytometric analysis of IgD expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either PerCp-Cy5.5 Rat IgG2a, kappa Isotype Control Cat. No. 550765; Left Panel or PerCP-Cy5.5 Rat Anti-Mouse IgD antibody Cat. No. 564273, Right Panel . Two-color contour plots showing the correlated expression of IgD or Ig isotype control staining and CD3e were derived from gated events with the forward and side light scattering characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IgD expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either PerCp-Cy5.5 Rat IgG2a, kappa Isotype Control Cat. No. 550765; Left Panel or PerCP-Cy5.5 Rat Anti-Mouse IgD antibody Cat. No. 564273, Right Panel . Two-color contour plots showing the correlated expression of IgD or Ig isotype control staining and CD3e were derived from gated events with the forward and side light scattering characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IgD expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either PerCp-Cy5.5 Rat IgG2a, kappa Isotype Control Cat. No. 550765; Left Panel or PerCP-Cy5.5 Rat Anti-Mouse IgD antibody Cat. No. 564273, Right Panel . Two-color contour plots showing the correlated expression of IgD or Ig isotype control staining and CD3e were derived from gated events with the forward and side light scattering characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IgD expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either PerCp-Cy5.5 Rat IgG2a, kappa Isotype Control Cat. No. 550765; Left Panel or PerCP-Cy5.5 Rat Anti-Mouse IgD antibody Cat. No. 564273, Right Panel . Two-color contour plots showing the correlated expression of IgD or Ig isotype control staining and CD3e were derived from gated events with the forward and side light scattering characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of IgD expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either PerCp-Cy5.5 Rat IgG2a, kappa Isotype Control Cat. No. 550765; Left Panel or PerCP-Cy5.5 Rat Anti-Mouse IgD antibody Cat. No. 564273, Right Panel . Two-color contour plots showing the correlated expression of IgD or Ig isotype control staining and CD3e were derived from gated events with the forward and side light scattering characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of IgD expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Hamster Anti-Mouse CD3e antibody Cat. No. 553066/561826 and either PerCp-Cy5.5 Rat IgG2a, kappa Isotype Control Cat. No. 550765; Left Panel or PerCP-Cy5.5 Rat Anti-Mouse IgD antibody Cat. No. 564273, Right Panel . Two-color contour plots showing the correlated expression of IgD or Ig isotype control staining and CD3e were derived from gated events with the forward and side light scattering characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.