BD PharmingenTM Purified Hamster Anti-Mouse CD120b

Description

Expression of cell surface p75 TNFR by BALB/c lymph node T cells. BALB/c lymph node cells were preincubated 15 min., 4C with purified 2.4G2 antibody rat anti-mouse CD16 FcgammaIII /CD32 FcgammaII ; Cat. No. 553142; 1 mug antibody/10e6 cells to block Fc receptor-mediated nonspecific staining by staining antibodies. The cells were stained 30 min., 4C with purified TR75-89 antibody 0.5 mug mAb/10e6 cells; Cat. No. 559916 . The cells were washed and were incubated 30 min., 4C with a biotin-conjugated cocktail of mouse anti-hamster antibodies Clones G70204 + G94-56; Cat. No. 554010; 0.5 mug mAb cocktail/10e6 cells . Finally, the cells were washed, were incubated with R-PE-conjugated streptavidin Cat. No. 554061; 0.015 mug PE-SA/10e6 cells and FITC-RA3-6B2/B220 Cat. No. 553088; 0.06 mug mAb/10e6 cells , and were washed in preparation for flow cytometric analysis with a FACScan Flow Cytometer. The immunofluorescent staining patterns for cells stained in the first stage with either purified TR75-89 antibody filled histogram or no antibody background staining; empty histogram followed by the 2nd- and 3rd-layer reagents are shown. The histograms were generated from reanalyzed flow cytometric data files that were gated for FITC-negative events with the light-scattering characteristics of lymphocytes, i.e., B220-negative lymph node T cells. Expression of cell surface p75 TNFR by BALB/c lymph node T cells. BALB/c lymph node cells were preincubated 15 min., 4C with purified 2.4G2 antibody rat anti-mouse CD16 FcgammaIII /CD32 FcgammaII ; Cat. No. 553142; 1 mug antibody/10e6 cells to block Fc receptor-mediated nonspecific staining by staining antibodies. The cells were stained 30 min., 4C with purified TR75-89 antibody 0.5 mug mAb/10e6 cells; Cat. No. 559916 . The cells were washed and were incubated 30 min., 4C with a biotin-conjugated cocktail of mouse anti-hamster antibodies Clones G70204 + G94-56; Cat. No. 554010; 0.5 mug mAb cocktail/10e6 cells . Finally, the cells were washed, were incubated with R-PE-conjugated streptavidin Cat. No. 554061; 0.015 mug PE-SA/10e6 cells and FITC-RA3-6B2/B220 Cat. No. 553088; 0.06 mug mAb/10e6 cells , and were washed in preparation for flow cytometric analysis with a FACScan Flow Cytometer. The immunofluorescent staining patterns for cells stained in the first stage with either purified TR75-89 antibody filled histogram or no antibody background staining; empty histogram followed by the 2nd- and 3rd-layer reagents are shown. The histograms were generated from reanalyzed flow cytometric data files that were gated for FITC-negative events with the light-scattering characteristics of lymphocytes, i.e., B220-negative lymph node T cells. Show More Expression of cell surface p75 TNFR by BALB/c lymph node T cells. BALB/c lymph node cells were preincubated 15 min., 4C with purified 2.4G2 antibody rat anti-mouse CD16 FcgammaIII /CD32 FcgammaII ; Cat. No. 553142; 1 mug antibody/10e6 cells to block Fc receptor-mediated nonspecific staining by staining antibodies. The cells were stained 30 min., 4C with purified TR75-89 antibody 0.5 mug mAb/10e6 cells; Cat. No. 559916 . The cells were washed and were incubated 30 min., 4C with a biotin-conjugated cocktail of mouse anti-hamster antibodies Clones G70204 + G94-56; Cat. No. 554010; 0.5 mug mAb cocktail/10e6 cells . Finally, the cells were washed, were incubated with R-PE-conjugated streptavidin Cat. No. 554061; 0.015 mug PE-SA/10e6 cells and FITC-RA3-6B2/B220 Cat. No. 553088; 0.06 mug mAb/10e6 cells , and were washed in preparation for flow cytometric analysis with a FACScan Flow Cytometer. The immunofluorescent staining patterns for cells stained in the first stage with either purified TR75-89 antibody filled histogram or no antibody background staining; empty histogram followed by the 2nd- and 3rd-layer reagents are shown. The histograms were generated from reanalyzed flow cytometric data files that were gated for FITC-negative events with the light-scattering characteristics of lymphocytes, i.e., B220-negative lymph node T cells.