BD PharmingenTM Purified Hamster Anti-Mouse CD152

Description

Expression of CD152 on activated T lymphocytes. C57BL/6 splenocytes were stained with either purified mAb UC10-4F10-11 open histogram or unstained filled histogram , in the presence of Mouse Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142 , followed by biotinylated anti-hamster IgG cocktail Cat. No. 554010 then Streptavidin-PE Cat. No. 554061 . The total viable leukocytes are displayed in the left panel. 48-hour ConA-activated C57BL/6 splenocytes were stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046/553047 , FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030/553031 , and purified mAb UC10-4F10-11 open histogram , or no purified antibody filled histogram , in the presence of Mouse Fc Block, followed by biotinylated anti-hamster IgG cocktail then Streptavidin-PE. The total viable T lymphocytes CD4+ and/or CD8a+ are displayed on the right panel. Flow cytometry was performed on a BD FACScan System BD Biosciences, San Jose, CA . Expression of CD152 on activated T lymphocytes. C57BL/6 splenocytes were stained with either purified mAb UC10-4F10-11 open histogram or unstained filled histogram , in the presence of Mouse Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142 , followed by biotinylated anti-hamster IgG cocktail Cat. No. 554010 then Streptavidin-PE Cat. No. 554061 . The total viable leukocytes are displayed in the left panel. 48-hour ConA-activated C57BL/6 splenocytes were stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046/553047 , FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030/553031 , and purified mAb UC10-4F10-11 open histogram , or no purified antibody filled histogram , in the presence of Mouse Fc Block, followed by biotinylated anti-hamster IgG cocktail then Streptavidin-PE. The total viable T lymphocytes CD4+ and/or CD8a+ are displayed on the right panel. Flow cytometry was performed on a BD FACScan System BD Biosciences, San Jose, CA . Expression of CD152 on activated T lymphocytes. C57BL/6 splenocytes were stained with either purified mAb UC10-4F10-11 open histogram or unstained filled histogram , in the presence of Mouse Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142 , followed by biotinylated anti-hamster IgG cocktail Cat. No. 554010 then Streptavidin-PE Cat. No. 554061 . The total viable leukocytes are displayed in the left panel. 48-hour ConA-activated C57BL/6 splenocytes were stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046/553047 , FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030/553031 , and purified mAb UC10-4F10-11 open histogram , or no purified antibody filled histogram , in the presence of Mouse Fc Block, followed by biotinylated anti-hamster IgG cocktail then Streptavidin-PE. The total viable T lymphocytes CD4+ and/or CD8a+ are displayed on the right panel. Flow cytometry was performed on a BD FACScan System BD Biosciences, San Jose, CA . Show More Expression of CD152 on activated T lymphocytes. C57BL/6 splenocytes were stained with either purified mAb UC10-4F10-11 open histogram or unstained filled histogram , in the presence of Mouse Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142 , followed by biotinylated anti-hamster IgG cocktail Cat. No. 554010 then Streptavidin-PE Cat. No. 554061 . The total viable leukocytes are displayed in the left panel. 48-hour ConA-activated C57BL/6 splenocytes were stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046/553047 , FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030/553031 , and purified mAb UC10-4F10-11 open histogram , or no purified antibody filled histogram , in the presence of Mouse Fc Block, followed by biotinylated anti-hamster IgG cocktail then Streptavidin-PE. The total viable T lymphocytes CD4+ and/or CD8a+ are displayed on the right panel. Flow cytometry was performed on a BD FACScan System BD Biosciences, San Jose, CA . Show More Expression of CD152 on activated T lymphocytes. C57BL/6 splenocytes were stained with either purified mAb UC10-4F10-11 open histogram or unstained filled histogram , in the presence of Mouse Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142 , followed by biotinylated anti-hamster IgG cocktail Cat. No. 554010 then Streptavidin-PE Cat. No. 554061 . The total viable leukocytes are displayed in the left panel. 48-hour ConA-activated C57BL/6 splenocytes were stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046/553047 , FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030/553031 , and purified mAb UC10-4F10-11 open histogram , or no purified antibody filled histogram , in the presence of Mouse Fc Block, followed by biotinylated anti-hamster IgG cocktail then Streptavidin-PE. The total viable T lymphocytes CD4+ and/or CD8a+ are displayed on the right panel. Flow cytometry was performed on a BD FACScan System BD Biosciences, San Jose, CA . Expression of CD152 on activated T lymphocytes. C57BL/6 splenocytes were stained with either purified mAb UC10-4F10-11 open histogram or unstained filled histogram , in the presence of Mouse Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142 , followed by biotinylated anti-hamster IgG cocktail Cat. No. 554010 then Streptavidin-PE Cat. No. 554061 . The total viable leukocytes are displayed in the left panel. 48-hour ConA-activated C57BL/6 splenocytes were stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046/553047 , FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030/553031 , and purified mAb UC10-4F10-11 open histogram , or no purified antibody filled histogram , in the presence of Mouse Fc Block, followed by biotinylated anti-hamster IgG cocktail then Streptavidin-PE. The total viable T lymphocytes CD4+ and/or CD8a+ are displayed on the right panel. Flow cytometry was performed on a BD FACScan System BD Biosciences, San Jose, CA .