BD PharmingenTM Purified Hamster Anti-Mouse CD154

Description

CD154 expression on activated and resting T lymphocytes. BALB/c spleen T cells, purified on a T Cell Enrichment Column R&D Systems , were cultured for 8 hours in the presence Panels A and C or absence Panel B of plate-bound 500A2 antibody anti-CD3e, Cat. No. 553238 . They were stained with purified MR1 antibody Panels A and B , followed by biotinylated mouse anti-hamster IgG, Cat. No. 554010, then Streptavidin-PE, Cat. No. 554061, Panels A, B, and C . Flow cytometry was performed on a BD FACScan Flow Cytometry System. CD154 expression on activated and resting T lymphocytes. BALB/c spleen T cells, purified on a T Cell Enrichment Column R&D Systems , were cultured for 8 hours in the presence Panels A and C or absence Panel B of plate-bound 500A2 antibody anti-CD3e, Cat. No. 553238 . They were stained with purified MR1 antibody Panels A and B , followed by biotinylated mouse anti-hamster IgG, Cat. No. 554010, then Streptavidin-PE, Cat. No. 554061, Panels A, B, and C . Flow cytometry was performed on a BD FACScan Flow Cytometry System. Show More CD154 expression on activated and resting T lymphocytes. BALB/c spleen T cells, purified on a T Cell Enrichment Column R&D Systems , were cultured for 8 hours in the presence Panels A and C or absence Panel B of plate-bound 500A2 antibody anti-CD3e, Cat. No. 553238 . They were stained with purified MR1 antibody Panels A and B , followed by biotinylated mouse anti-hamster IgG, Cat. No. 554010, then Streptavidin-PE, Cat. No. 554061, Panels A, B, and C . Flow cytometry was performed on a BD FACScan Flow Cytometry System.