BD PharmingenTM Purified Hamster Anti-Mouse CD28

Description

Two-color analysis of CD28 expression of splenic T lymphocytes. BALB/c splenocytes were preincubated with purified anti-mouse CD16/CD32 mAb 2.4G2 Mouse BD Fc Block Cat. No. 553141 , then stained simultaneously with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046 and FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030 in addition to staining with purified anti-mouse CD28 clone 37.51 right panel . Staining of CD28-positive cells was detected with biotinylated anti-Syrian hamster IgG2 mAb G192-3 Cat. No. 554029 , followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a BD FACScan flow cytometry system. Two-color analysis of CD28 expression of splenic T lymphocytes. BALB/c splenocytes were preincubated with purified anti-mouse CD16/CD32 mAb 2.4G2 Mouse BD Fc Block Cat. No. 553141 , then stained simultaneously with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046 and FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030 in addition to staining with purified anti-mouse CD28 clone 37.51 right panel . Staining of CD28-positive cells was detected with biotinylated anti-Syrian hamster IgG2 mAb G192-3 Cat. No. 554029 , followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a BD FACScan flow cytometry system. Two-color analysis of CD28 expression of splenic T lymphocytes. BALB/c splenocytes were preincubated with purified anti-mouse CD16/CD32 mAb 2.4G2 Mouse BD Fc Block Cat. No. 553141 , then stained simultaneously with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046 and FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030 in addition to staining with purified anti-mouse CD28 clone 37.51 right panel . Staining of CD28-positive cells was detected with biotinylated anti-Syrian hamster IgG2 mAb G192-3 Cat. No. 554029 , followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a BD FACScan flow cytometry system. Show More Two-color analysis of CD28 expression of splenic T lymphocytes. BALB/c splenocytes were preincubated with purified anti-mouse CD16/CD32 mAb 2.4G2 Mouse BD Fc Block Cat. No. 553141 , then stained simultaneously with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046 and FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030 in addition to staining with purified anti-mouse CD28 clone 37.51 right panel . Staining of CD28-positive cells was detected with biotinylated anti-Syrian hamster IgG2 mAb G192-3 Cat. No. 554029 , followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a BD FACScan flow cytometry system. Show More Two-color analysis of CD28 expression of splenic T lymphocytes. BALB/c splenocytes were preincubated with purified anti-mouse CD16/CD32 mAb 2.4G2 Mouse BD Fc Block Cat. No. 553141 , then stained simultaneously with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046 and FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030 in addition to staining with purified anti-mouse CD28 clone 37.51 right panel . Staining of CD28-positive cells was detected with biotinylated anti-Syrian hamster IgG2 mAb G192-3 Cat. No. 554029 , followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a BD FACScan flow cytometry system. Two-color analysis of CD28 expression of splenic T lymphocytes. BALB/c splenocytes were preincubated with purified anti-mouse CD16/CD32 mAb 2.4G2 Mouse BD Fc Block Cat. No. 553141 , then stained simultaneously with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046 and FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030 in addition to staining with purified anti-mouse CD28 clone 37.51 right panel . Staining of CD28-positive cells was detected with biotinylated anti-Syrian hamster IgG2 mAb G192-3 Cat. No. 554029 , followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a BD FACScan flow cytometry system.