BD PharmingenTM Purified Mouse anti-Akt (pS473)

Description

LEFT: Western blot analysis of AKT pS473 in mouse embryonic fibroblasts. Lysates from control left blot and PDGF-treated Sigma Cat. No. P8147, right blot NIH/3T3 cells ATCC CRL-1658 were probed with Purified Mouse anti-Akt pS473 monoclonal antibody at the following concentrations: 12.5 lanes 1 , 6.25 lanes 2 , and 3.125 ng/ml lanes 3 . AKT pS473 is identified as a 60-kDa band in the treated cells. RIGHT: Western blot analysis of AKT pS473 in human T leukemia. Lysates from control left panel and Wortmannin-treated Invitrogen, Cat. No. PHZ1301, right panel Jurkat cells ATCC TIB152 were probed with Purified Mouse anti-Akt pS473 monoclonal antibody at the following concentrations: 2.0 lanes 1 , 1.0 lanes 2 , and 0.5 mug/ml lanes 3 . The data demonstrates that the level of phosphorylation of Akt pS473 decreases when phosphatidylinositol 3-kinase activity is inhibited. LEFT: Western blot analysis of AKT pS473 in mouse embryonic fibroblasts. Lysates from control left blot and PDGF-treated Sigma Cat. No. P8147, right blot NIH/3T3 cells ATCC CRL-1658 were probed with Purified Mouse anti-Akt pS473 monoclonal antibody at the following concentrations: 12.5 lanes 1 , 6.25 lanes 2 , and 3.125 ng/ml lanes 3 . AKT pS473 is identified as a 60-kDa band in the treated cells. RIGHT: Western blot analysis of AKT pS473 in human T leukemia. Lysates from control left panel and Wortmannin-treated Invitrogen, Cat. No. PHZ1301, right panel Jurkat cells ATCC TIB152 were probed with Purified Mouse anti-Akt pS473 monoclonal antibody at the following concentrations: 2.0 lanes 1 , 1.0 lanes 2 , and 0.5 mug/ml lanes 3 . The data demonstrates that the level of phosphorylation of Akt pS473 decreases when phosphatidylinositol 3-kinase activity is inhibited. Show More LEFT: Western blot analysis of AKT pS473 in mouse embryonic fibroblasts. Lysates from control left blot and PDGF-treated Sigma Cat. No. P8147, right blot NIH/3T3 cells ATCC CRL-1658 were probed with Purified Mouse anti-Akt pS473 monoclonal antibody at the following concentrations: 12.5 lanes 1 , 6.25 lanes 2 , and 3.125 ng/ml lanes 3 . AKT pS473 is identified as a 60-kDa band in the treated cells. RIGHT: Western blot analysis of AKT pS473 in human T leukemia. Lysates from control left panel and Wortmannin-treated Invitrogen, Cat. No. PHZ1301, right panel Jurkat cells ATCC TIB152 were probed with Purified Mouse anti-Akt pS473 monoclonal antibody at the following concentrations: 2.0 lanes 1 , 1.0 lanes 2 , and 0.5 mug/ml lanes 3 . The data demonstrates that the level of phosphorylation of Akt pS473 decreases when phosphatidylinositol 3-kinase activity is inhibited.