BD PharmingenTM Purified Mouse Anti-ASGPR 1

Description

Flow Cytometric Analysis of ASGPR 1 expression in Human Hepatocellular Carcinoma Hep G2 cells. Hep G2 cells ATCC, HB-8065 were harvested using BD Accutase Cell Detachment Solution Cat. No. 561527 . The cells were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-ASGPR 1 antibody Cat. No. 563654; solid line histogram at matched concentrations. The second-step reagent was PE Goat Anti-Mouse Ig Cat. No. 550589 . Histograms were derived from gated events based on light-scattering characteristics of viable Hep G2 cells. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometry System. Immunofluorescent Staining of ASGPR 1 in Human Hepatocellular Carcinoma Hep G2 cells. Hep G2 cells ATCC, HB-8065 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and stained with Purified Mouse Anti-ASGPR 1 pseudo-colored green at 5 mug/mL. The second-step reagent was Alexa Fluor 488 Goat Anti-Mouse IgG Life Technologies , and counter-staining was with DAPI 4,6-Diamidino-2-phenylindole dihydrochloride; pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD AttoVision Software. Flow Cytometric Analysis of ASGPR 1 expression in Human Hepatocellular Carcinoma Hep G2 cells. Hep G2 cells ATCC, HB-8065 were harvested using BD Accutase Cell Detachment Solution Cat. No. 561527 . The cells were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-ASGPR 1 antibody Cat. No. 563654; solid line histogram at matched concentrations. The second-step reagent was PE Goat Anti-Mouse Ig Cat. No. 550589 . Histograms were derived from gated events based on light-scattering characteristics of viable Hep G2 cells. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometry System. Show More Immunofluorescent Staining of ASGPR 1 in Human Hepatocellular Carcinoma Hep G2 cells. Hep G2 cells ATCC, HB-8065 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and stained with Purified Mouse Anti-ASGPR 1 pseudo-colored green at 5 mug/mL. The second-step reagent was Alexa Fluor 488 Goat Anti-Mouse IgG Life Technologies , and counter-staining was with DAPI 4,6-Diamidino-2-phenylindole dihydrochloride; pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD AttoVision Software. Show More Flow Cytometric Analysis of ASGPR 1 expression in Human Hepatocellular Carcinoma Hep G2 cells. Hep G2 cells ATCC, HB-8065 were harvested using BD Accutase Cell Detachment Solution Cat. No. 561527 . The cells were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-ASGPR 1 antibody Cat. No. 563654; solid line histogram at matched concentrations. The second-step reagent was PE Goat Anti-Mouse Ig Cat. No. 550589 . Histograms were derived from gated events based on light-scattering characteristics of viable Hep G2 cells. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometry System. Immunofluorescent Staining of ASGPR 1 in Human Hepatocellular Carcinoma Hep G2 cells. Hep G2 cells ATCC, HB-8065 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and stained with Purified Mouse Anti-ASGPR 1 pseudo-colored green at 5 mug/mL. The second-step reagent was Alexa Fluor 488 Goat Anti-Mouse IgG Life Technologies , and counter-staining was with DAPI 4,6-Diamidino-2-phenylindole dihydrochloride; pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD AttoVision Software.