BD PharmingenTM Purified Mouse anti-Bcl-6

Description

Analysis of Bcl-6 expression by Western blot and Immunohistochemistry. Left Panel: Western blot analysis of Bcl-6 expressed by Human Jurkat Cat. No. 611451 and Ramos cell lines. Cell lysates from untreated Jurkat lanes 1-5 and Ramos lanes 6-10 cells 15 mug total cellular protein/lane were electrophoresed SDS-PAGE , transferred to membranes and then probed with Purified Mouse Anti-Bcl-6 antibody Clone K112-91; Cat. No. 561520 at concentrations of 2 lanes 1, 6 , 0.667 lanes 2, 7 , 0.222 lanes 3, 8 , 0.074 lanes 4, 9 and 0.025 lanes 5, 10 mug/ml. Bcl-6 is identified as a band of 87-98 kDa in the Ramos cell lysate. Middle Panel: Cell lysates from untreated Mouse BA/F3 lanes 1-5 and A20 lanes 6-10 cells 15 mug total cellular protein/lane were electrophoresed SDS-PAGE , transferred to membranes and then probed with Purified Mouse Anti-Bcl-6 Clone K112-91; Cat. No. 561520 antibody at concentrations of 2 lanes 1, 6 , 0.667 lanes 2, 7 , 0.222 lanes 3, 8 , 0.074 lanes 4, 9 , and 0.025 lanes 5, 10 mug/ml. Bcl-6 is identified as a band of 87-98 kDa in the A20 cell lysate. Right Panel: Bcl-6 staining of human tonsil. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 550878; Left Image or Purified Mouse Anti-Bcl-6 antibody Clone K112-91; Cat. No. 561520; Middle and Right Images , with Hematoxylin counterstaining. Bcl-6 is detected in the nuclei of the lymphocytes within the lymphoid follicles of the tonsil. Original magnifications: 20X and 40X as shown. Analysis of Bcl-6 expression by Western blot and Immunohistochemistry. Left Panel: Western blot analysis of Bcl-6 expressed by Human Jurkat Cat. No. 611451 and Ramos cell lines. Cell lysates from untreated Jurkat lanes 1-5 and Ramos lanes 6-10 cells 15 mug total cellular protein/lane were electrophoresed SDS-PAGE , transferred to membranes and then probed with Purified Mouse Anti-Bcl-6 antibody Clone K112-91; Cat. No. 561520 at concentrations of 2 lanes 1, 6 , 0.667 lanes 2, 7 , 0.222 lanes 3, 8 , 0.074 lanes 4, 9 and 0.025 lanes 5, 10 mug/ml. Bcl-6 is identified as a band of 87-98 kDa in the Ramos cell lysate. Middle Panel: Cell lysates from untreated Mouse BA/F3 lanes 1-5 and A20 lanes 6-10 cells 15 mug total cellular protein/lane were electrophoresed SDS-PAGE , transferred to membranes and then probed with Purified Mouse Anti-Bcl-6 Clone K112-91; Cat. No. 561520 antibody at concentrations of 2 lanes 1, 6 , 0.667 lanes 2, 7 , 0.222 lanes 3, 8 , 0.074 lanes 4, 9 , and 0.025 lanes 5, 10 mug/ml. Bcl-6 is identified as a band of 87-98 kDa in the A20 cell lysate. Right Panel: Bcl-6 staining of human tonsil. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 550878; Left Image or Purified Mouse Anti-Bcl-6 antibody Clone K112-91; Cat. No. 561520; Middle and Right Images , with Hematoxylin counterstaining. Bcl-6 is detected in the nuclei of the lymphocytes within the lymphoid follicles of the tonsil. Original magnifications: 20X and 40X as shown. Show More Analysis of Bcl-6 expression by Western blot and Immunohistochemistry. Left Panel: Western blot analysis of Bcl-6 expressed by Human Jurkat Cat. No. 611451 and Ramos cell lines. Cell lysates from untreated Jurkat lanes 1-5 and Ramos lanes 6-10 cells 15 mug total cellular protein/lane were electrophoresed SDS-PAGE , transferred to membranes and then probed with Purified Mouse Anti-Bcl-6 antibody Clone K112-91; Cat. No. 561520 at concentrations of 2 lanes 1, 6 , 0.667 lanes 2, 7 , 0.222 lanes 3, 8 , 0.074 lanes 4, 9 and 0.025 lanes 5, 10 mug/ml. Bcl-6 is identified as a band of 87-98 kDa in the Ramos cell lysate. Middle Panel: Cell lysates from untreated Mouse BA/F3 lanes 1-5 and A20 lanes 6-10 cells 15 mug total cellular protein/lane were electrophoresed SDS-PAGE , transferred to membranes and then probed with Purified Mouse Anti-Bcl-6 Clone K112-91; Cat. No. 561520 antibody at concentrations of 2 lanes 1, 6 , 0.667 lanes 2, 7 , 0.222 lanes 3, 8 , 0.074 lanes 4, 9 , and 0.025 lanes 5, 10 mug/ml. Bcl-6 is identified as a band of 87-98 kDa in the A20 cell lysate. Right Panel: Bcl-6 staining of human tonsil. Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 550878; Left Image or Purified Mouse Anti-Bcl-6 antibody Clone K112-91; Cat. No. 561520; Middle and Right Images , with Hematoxylin counterstaining. Bcl-6 is detected in the nuclei of the lymphocytes within the lymphoid follicles of the tonsil. Original magnifications: 20X and 40X as shown.