BD PharmingenTM Purified Mouse Anti-Cyclin B1

Description

Left Figure: Western blot analysis of cyclin B1. Lane 1: K562 human leukemia cell lysate. Lane 2: 293 human embryonic kidney cell lysate. Anti-human cyclin B1 Cat. No. 554176 identifies cyclin B1 as an 62 kDa band. Right Figure: Cyclin B1 staining of U-2 OS ATCC HTB-96 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-cyclin B1 antibody. The second step reagent was Alexa Fluor 488 goat anti mouse Ig Invitrogen . Images were taken on a BD Pathway 855 Bioimager system using a 20x objective. This antibody also stained A549 ATCC CCL-185 and HeLa CCL-2 cells and worked with both the Triton X-100 and alcohol perm protocols see Recommended Assay Procedure . Left Figure: Western blot analysis of cyclin B1. Lane 1: K562 human leukemia cell lysate. Lane 2: 293 human embryonic kidney cell lysate. Anti-human cyclin B1 Cat. No. 554176 identifies cyclin B1 as an 62 kDa band. Right Figure: Cyclin B1 staining of U-2 OS ATCC HTB-96 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-cyclin B1 antibody. The second step reagent was Alexa Fluor 488 goat anti mouse Ig Invitrogen . Images were taken on a BD Pathway 855 Bioimager system using a 20x objective. This antibody also stained A549 ATCC CCL-185 and HeLa CCL-2 cells and worked with both the Triton X-100 and alcohol perm protocols see Recommended Assay Procedure . Left Figure: Western blot analysis of cyclin B1. Lane 1: K562 human leukemia cell lysate. Lane 2: 293 human embryonic kidney cell lysate. Anti-human cyclin B1 Cat. No. 554176 identifies cyclin B1 as an 62 kDa band. Right Figure: Cyclin B1 staining of U-2 OS ATCC HTB-96 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-cyclin B1 antibody. The second step reagent was Alexa Fluor 488 goat anti mouse Ig Invitrogen . Images were taken on a BD Pathway 855 Bioimager system using a 20x objective. This antibody also stained A549 ATCC CCL-185 and HeLa CCL-2 cells and worked with both the Triton X-100 and alcohol perm protocols see Recommended Assay Procedure . Show More Left Figure: Western blot analysis of cyclin B1. Lane 1: K562 human leukemia cell lysate. Lane 2: 293 human embryonic kidney cell lysate. Anti-human cyclin B1 Cat. No. 554176 identifies cyclin B1 as an 62 kDa band. Right Figure: Cyclin B1 staining of U-2 OS ATCC HTB-96 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-cyclin B1 antibody. The second step reagent was Alexa Fluor 488 goat anti mouse Ig Invitrogen . Images were taken on a BD Pathway 855 Bioimager system using a 20x objective. This antibody also stained A549 ATCC CCL-185 and HeLa CCL-2 cells and worked with both the Triton X-100 and alcohol perm protocols see Recommended Assay Procedure . Show More Left Figure: Western blot analysis of cyclin B1. Lane 1: K562 human leukemia cell lysate. Lane 2: 293 human embryonic kidney cell lysate. Anti-human cyclin B1 Cat. No. 554176 identifies cyclin B1 as an 62 kDa band. Right Figure: Cyclin B1 staining of U-2 OS ATCC HTB-96 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-cyclin B1 antibody. The second step reagent was Alexa Fluor 488 goat anti mouse Ig Invitrogen . Images were taken on a BD Pathway 855 Bioimager system using a 20x objective. This antibody also stained A549 ATCC CCL-185 and HeLa CCL-2 cells and worked with both the Triton X-100 and alcohol perm protocols see Recommended Assay Procedure . Left Figure: Western blot analysis of cyclin B1. Lane 1: K562 human leukemia cell lysate. Lane 2: 293 human embryonic kidney cell lysate. Anti-human cyclin B1 Cat. No. 554176 identifies cyclin B1 as an 62 kDa band. Right Figure: Cyclin B1 staining of U-2 OS ATCC HTB-96 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-cyclin B1 antibody. The second step reagent was Alexa Fluor 488 goat anti mouse Ig Invitrogen . Images were taken on a BD Pathway 855 Bioimager system using a 20x objective. This antibody also stained A549 ATCC CCL-185 and HeLa CCL-2 cells and worked with both the Triton X-100 and alcohol perm protocols see Recommended Assay Procedure .