BD PharmingenTM Purified Mouse Anti- Cyclin B1

Description

Western blot analysis of Cyclin B1. Lysate from Jurkat T cells was probed with anti- Cyclin B1 antibody at 2 mug/ml. Cyclin B1 is identified as an 62 kDa band. Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10 000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-Cyclin B1 antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . Images were taken on a BD Pathway 855 Bioimager system using a 20x objective. This antibody also stained A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells and worked with both the Triton X-100 and alcohol perm protocols see Recommended Assay Procedure . Western blot analysis of Cyclin B1. Lysate from Jurkat T cells was probed with anti- Cyclin B1 antibody at 2 mug/ml. Cyclin B1 is identified as an 62 kDa band. Show More Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10 000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-Cyclin B1 antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . Images were taken on a BD Pathway 855 Bioimager system using a 20x objective. This antibody also stained A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells and worked with both the Triton X-100 and alcohol perm protocols see Recommended Assay Procedure . Show More Western blot analysis of Cyclin B1. Lysate from Jurkat T cells was probed with anti- Cyclin B1 antibody at 2 mug/ml. Cyclin B1 is identified as an 62 kDa band. Immunofluorescent staining of HeLa ATCC CCL-2 cells. Cells were seeded in a 96 well imaging plate Cat. No. 353219 at 10 000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-Cyclin B1 antibody. The second step reagent was FITC goat anti mouse Ig Cat. No. 554001 . Images were taken on a BD Pathway 855 Bioimager system using a 20x objective. This antibody also stained A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells and worked with both the Triton X-100 and alcohol perm protocols see Recommended Assay Procedure .