BD PharmingenTM Purified Mouse anti-Human Alkaline Phosphatase

Description

Flow cytometric analysis of Alkaline Phosphatase expression on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI grown in mTeSR1 medium StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were harvested and stained with Purified Mouse anti-Human Alkaline Phosphatase antibody solid line or a Purified mouse IgG1, kappa isotype control Clone MOPC-21, Cat. No. 556648, dashed line . The second step reagent was FITC goat anti-mouse Ig Cat. No. 554001 . Flow cytometry was performed on a BD LSR II flow cytometry system. Immunoflourescent staining of Alkaline Phosphatase on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI passage 52 grown in mTeSR1 medium StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and stained with Purified Mouse anti-Human Alkaline Phosphatase monoclonal antibody pseudo colored green at 5 mug/mL. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies and counter-staining of cell nuclei was with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Flow cytometric analysis of Alkaline Phosphatase expression on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI grown in mTeSR1 medium StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were harvested and stained with Purified Mouse anti-Human Alkaline Phosphatase antibody solid line or a Purified mouse IgG1, kappa isotype control Clone MOPC-21, Cat. No. 556648, dashed line . The second step reagent was FITC goat anti-mouse Ig Cat. No. 554001 . Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Immunoflourescent staining of Alkaline Phosphatase on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI passage 52 grown in mTeSR1 medium StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and stained with Purified Mouse anti-Human Alkaline Phosphatase monoclonal antibody pseudo colored green at 5 mug/mL. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies and counter-staining of cell nuclei was with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Show More Flow cytometric analysis of Alkaline Phosphatase expression on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI grown in mTeSR1 medium StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were harvested and stained with Purified Mouse anti-Human Alkaline Phosphatase antibody solid line or a Purified mouse IgG1, kappa isotype control Clone MOPC-21, Cat. No. 556648, dashed line . The second step reagent was FITC goat anti-mouse Ig Cat. No. 554001 . Flow cytometry was performed on a BD LSR II flow cytometry system. Immunoflourescent staining of Alkaline Phosphatase on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI passage 52 grown in mTeSR1 medium StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and stained with Purified Mouse anti-Human Alkaline Phosphatase monoclonal antibody pseudo colored green at 5 mug/mL. The second-step reagent was Alexa Fluor 488 goat anti-mouse Ig Life Technologies and counter-staining of cell nuclei was with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software.