BD PharmingenTM Purified Mouse Anti-Human Caspase-8

Description

Western blot analysis of caspase-8. Lysates from control lanes 1-3 and camptothecin treated Jurkat cells lanes 4-6 were probed with anti-human caspase-8 clone 3-1-9, Cat. No. 551243 at the following concentrations: 0.5 lanes 1,4 , 0.25 lanes 2,5 , and 0.125 mug/ml lanes 3,6 . Caspase-8 is identified as 55/50 kDa proform , 40/36 kDa cleaved , and 23 kDa cleaved bands in treated cells and the 55 kDa band in control cells. + =positive, - =negative, NT =not tested Immunoprecipitation/western blot analysis of caspase-8. Lysates from control lane 1 or camptothecin-treated Jurkat cells lane 2 were each immunoprecipitated with anti-human caspase-8 clone 3-1-9 , and western blotted with anti-human caspase-8 clone 3-1-9 . The 55 kDa proform caspase-8 was identified in both control and camptothecin-treated cells. Western blot analysis of caspase-8. Lysates from control lanes 1-3 and camptothecin treated Jurkat cells lanes 4-6 were probed with anti-human caspase-8 clone 3-1-9, Cat. No. 551243 at the following concentrations: 0.5 lanes 1,4 , 0.25 lanes 2,5 , and 0.125 mug/ml lanes 3,6 . Caspase-8 is identified as 55/50 kDa proform , 40/36 kDa cleaved , and 23 kDa cleaved bands in treated cells and the 55 kDa band in control cells. Show More + =positive, - =negative, NT =not tested Show More Immunoprecipitation/western blot analysis of caspase-8. Lysates from control lane 1 or camptothecin-treated Jurkat cells lane 2 were each immunoprecipitated with anti-human caspase-8 clone 3-1-9 , and western blotted with anti-human caspase-8 clone 3-1-9 . The 55 kDa proform caspase-8 was identified in both control and camptothecin-treated cells. Show More Western blot analysis of caspase-8. Lysates from control lanes 1-3 and camptothecin treated Jurkat cells lanes 4-6 were probed with anti-human caspase-8 clone 3-1-9, Cat. No. 551243 at the following concentrations: 0.5 lanes 1,4 , 0.25 lanes 2,5 , and 0.125 mug/ml lanes 3,6 . Caspase-8 is identified as 55/50 kDa proform , 40/36 kDa cleaved , and 23 kDa cleaved bands in treated cells and the 55 kDa band in control cells. + =positive, - =negative, NT =not tested Immunoprecipitation/western blot analysis of caspase-8. Lysates from control lane 1 or camptothecin-treated Jurkat cells lane 2 were each immunoprecipitated with anti-human caspase-8 clone 3-1-9 , and western blotted with anti-human caspase-8 clone 3-1-9 . The 55 kDa proform caspase-8 was identified in both control and camptothecin-treated cells.