BD PharmingenTM Purified Mouse Anti-Human CCR7 (CD197)

Description

Multicolor flow cytometric analysis of CCR7 CD197 expression on human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were washed and then stained with either purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or purified Mouse Anti-Human CCR7 CD197 antibody Cat. No. 567209; Right Plot at 0.25 mug/test. After washing, the cells were stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . After blocking with Normal Mouse Serum ThermoFisher, Cat. No. 10410 , the cells were further stained with Alexa Fluor647 Mouse Anti-Human CD4 Cat. No. 557707 and BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 antibodies. The two-color flow cytometric contour plot showing the correlated expression of CD45RA versus CCR7 CD197 or Ig Isotype control staining was derived from CD4-positive T cell-gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSCanto II Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Immunohistochemical analysis of CCR7 CD197 expression by cells within human tonsil. Cold acetone-fixed frozen sections of human tonsil were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 550878; Left Panel or Purified Mouse Anti-Human CCR7 CD197 antibody Cat. No. 567209; Right Panel at 5 mug/ml for one hour at room temperature. A three-step staining procedure that employs a Biotin Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550337 , Streptavidin-Horseradish Peroxidase Sav-HRP Cat. No. 550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. The tissues were counterstained with hematoxylin. CCR7 CD197 positive staining on tonsillar cells Right Panel is indicated by the intense brown labeling. Original magnification: 20X. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CCR7 CD197 expression on human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were washed and then stained with either purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or purified Mouse Anti-Human CCR7 CD197 antibody Cat. No. 567209; Right Plot at 0.25 mug/test. After washing, the cells were stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . After blocking with Normal Mouse Serum ThermoFisher, Cat. No. 10410 , the cells were further stained with Alexa Fluor647 Mouse Anti-Human CD4 Cat. No. 557707 and BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 antibodies. The two-color flow cytometric contour plot showing the correlated expression of CD45RA versus CCR7 CD197 or Ig Isotype control staining was derived from CD4-positive T cell-gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSCanto II Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Immunohistochemical analysis of CCR7 CD197 expression by cells within human tonsil. Cold acetone-fixed frozen sections of human tonsil were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 550878; Left Panel or Purified Mouse Anti-Human CCR7 CD197 antibody Cat. No. 567209; Right Panel at 5 mug/ml for one hour at room temperature. A three-step staining procedure that employs a Biotin Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550337 , Streptavidin-Horseradish Peroxidase Sav-HRP Cat. No. 550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. The tissues were counterstained with hematoxylin. CCR7 CD197 positive staining on tonsillar cells Right Panel is indicated by the intense brown labeling. Original magnification: 20X. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CCR7 CD197 expression on human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leucocytes were washed and then stained with either purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or purified Mouse Anti-Human CCR7 CD197 antibody Cat. No. 567209; Right Plot at 0.25 mug/test. After washing, the cells were stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . After blocking with Normal Mouse Serum ThermoFisher, Cat. No. 10410 , the cells were further stained with Alexa Fluor647 Mouse Anti-Human CD4 Cat. No. 557707 and BD Horizon BV421 Mouse Anti-Human CD45RA Cat. No. 562885 antibodies. The two-color flow cytometric contour plot showing the correlated expression of CD45RA versus CCR7 CD197 or Ig Isotype control staining was derived from CD4-positive T cell-gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSCanto II Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Immunohistochemical analysis of CCR7 CD197 expression by cells within human tonsil. Cold acetone-fixed frozen sections of human tonsil were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 550878; Left Panel or Purified Mouse Anti-Human CCR7 CD197 antibody Cat. No. 567209; Right Panel at 5 mug/ml for one hour at room temperature. A three-step staining procedure that employs a Biotin Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550337 , Streptavidin-Horseradish Peroxidase Sav-HRP Cat. No. 550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. The tissues were counterstained with hematoxylin. CCR7 CD197 positive staining on tonsillar cells Right Panel is indicated by the intense brown labeling. Original magnification: 20X. Data shown on this Technical Data Sheet are not lot specific.