BD PharmingenTM Purified Mouse Anti-Human CD124

Description

Flow cytometric analysis of CD124 expression on human PBMC. Human PBMC isolated by density gradient centrifugation Ficoll-Paque were blocked with normal polyclonal human IgG and stained with either Purified Mouse Anti-Human CD124 Cat. No. 551894; filled histogram or Purified Mouse IgG1, kappa Isotype Control Cat. No. 555746; open histogram , followed by Biotin Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 553999 and PE Streptavidin Cat. No. 554061 . Fluorescence histograms depicting CD124 or Ig isotype expression were derived from gated events with the forward and side light-scatter characteristics of viable CD19+ lymphocytes. Note: Certain human cell lines or cell types e.g., neutrophils and monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Flow cytometric analysis of CD124 expression on human PBMC. Human PBMC isolated by density gradient centrifugation Ficoll-Paque were blocked with normal polyclonal human IgG and stained with either Purified Mouse Anti-Human CD124 Cat. No. 551894; filled histogram or Purified Mouse IgG1, kappa Isotype Control Cat. No. 555746; open histogram , followed by Biotin Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 553999 and PE Streptavidin Cat. No. 554061 . Fluorescence histograms depicting CD124 or Ig isotype expression were derived from gated events with the forward and side light-scatter characteristics of viable CD19+ lymphocytes. Note: Certain human cell lines or cell types e.g., neutrophils and monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors. Show More Flow cytometric analysis of CD124 expression on human PBMC. Human PBMC isolated by density gradient centrifugation Ficoll-Paque were blocked with normal polyclonal human IgG and stained with either Purified Mouse Anti-Human CD124 Cat. No. 551894; filled histogram or Purified Mouse IgG1, kappa Isotype Control Cat. No. 555746; open histogram , followed by Biotin Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 553999 and PE Streptavidin Cat. No. 554061 . Fluorescence histograms depicting CD124 or Ig isotype expression were derived from gated events with the forward and side light-scatter characteristics of viable CD19+ lymphocytes. Note: Certain human cell lines or cell types e.g., neutrophils and monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.