BD PharmingenTM Purified Mouse Anti-Human CD126

Description

Expression of cell surface IL-6R by human PBMC. Human PBMC isolated by density centrifugation Ficoll-Paque were blocked with normal polyclonal human IgG and stained with Purified Mouse Anti-Human CD126 Cat. No. 551462 followed by Biotin Rat Anti-Mouse IgG1 Cat. No. 553441 and PE Streptavidin Cat. No. 554061 in a three-layer staining protocol to amplify immunofluorescent signals. Staining with the Purified Mouse Anti-Human CD126 filled histograms is compared to staining obtained using Purified Mouse IgG1, kappa Isotype Control Cat. No. 555746; open histograms . Histogram in figure is gated on the CD19 lymphocytes. Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors for example see Browning et al . Expression of cell surface IL-6R by human PBMC. Human PBMC isolated by density centrifugation Ficoll-Paque were blocked with normal polyclonal human IgG and stained with Purified Mouse Anti-Human CD126 Cat. No. 551462 followed by Biotin Rat Anti-Mouse IgG1 Cat. No. 553441 and PE Streptavidin Cat. No. 554061 in a three-layer staining protocol to amplify immunofluorescent signals. Staining with the Purified Mouse Anti-Human CD126 filled histograms is compared to staining obtained using Purified Mouse IgG1, kappa Isotype Control Cat. No. 555746; open histograms . Histogram in figure is gated on the CD19 lymphocytes. Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors for example see Browning et al . Show More Expression of cell surface IL-6R by human PBMC. Human PBMC isolated by density centrifugation Ficoll-Paque were blocked with normal polyclonal human IgG and stained with Purified Mouse Anti-Human CD126 Cat. No. 551462 followed by Biotin Rat Anti-Mouse IgG1 Cat. No. 553441 and PE Streptavidin Cat. No. 554061 in a three-layer staining protocol to amplify immunofluorescent signals. Staining with the Purified Mouse Anti-Human CD126 filled histograms is compared to staining obtained using Purified Mouse IgG1, kappa Isotype Control Cat. No. 555746; open histograms . Histogram in figure is gated on the CD19 lymphocytes. Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors for example see Browning et al .