BD PharmingenTM Purified Mouse Anti-Human CD157

Description

Multiparameter flow cytometric analysis of CD157 expression on Human peripheral blood leukocyte populations. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leukocytes were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 555746; Left Plot or Purified Mouse Anti-Human CD157 antibody Cat. No. 569643; Right Plot at 0.125 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption antibody Cat. No. 550589 . The bivariate pseudocolor density plot showing the correlated expression of CD157 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD157 expression on Human peripheral blood leukocyte populations. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leukocytes were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 555746; Left Plot or Purified Mouse Anti-Human CD157 antibody Cat. No. 569643; Right Plot at 0.125 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption antibody Cat. No. 550589 . The bivariate pseudocolor density plot showing the correlated expression of CD157 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD157 expression on Human peripheral blood leukocyte populations. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leukocytes were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 555746; Left Plot or Purified Mouse Anti-Human CD157 antibody Cat. No. 569643; Right Plot at 0.125 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption antibody Cat. No. 550589 . The bivariate pseudocolor density plot showing the correlated expression of CD157 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD157 expression on Human peripheral blood leukocyte populations. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leukocytes were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 555746; Left Plot or Purified Mouse Anti-Human CD157 antibody Cat. No. 569643; Right Plot at 0.125 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption antibody Cat. No. 550589 . The bivariate pseudocolor density plot showing the correlated expression of CD157 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD157 expression on Human peripheral blood leukocyte populations. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leukocytes were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 555746; Left Plot or Purified Mouse Anti-Human CD157 antibody Cat. No. 569643; Right Plot at 0.125 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption antibody Cat. No. 550589 . The bivariate pseudocolor density plot showing the correlated expression of CD157 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD157 expression on Human peripheral blood leukocyte populations. Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 . The leukocytes were stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 555746; Left Plot or Purified Mouse Anti-Human CD157 antibody Cat. No. 569643; Right Plot at 0.125 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption antibody Cat. No. 550589 . The bivariate pseudocolor density plot showing the correlated expression of CD157 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.