BD PharmingenTM Purified Mouse Anti-Human CD178

Description

Western blot analysis of Fas Ligand FasL . L5187Y human T lymphoma cells were transfected with human FasL cDNA and then not treated lane 1 or treated lane 2 with the metalloproteinase inhibitor, KB8301. KB8301 blocks FasL cleavage from the cell surface, resulting in high levels membrane expressed FasL. The blot shows that whereas FasL is detected in untreated cells lane 1 , levels increased dramatically when cells were treated with KB8301 lane 2 . A mouse IgG1 isotype matched antibody was used as a negative control lane 3 . Western blot analysis of Fas Ligand FasL . L5187Y human T lymphoma cells were transfected with human FasL cDNA and then not treated lane 1 or treated lane 2 with the metalloproteinase inhibitor, KB8301. KB8301 blocks FasL cleavage from the cell surface, resulting in high levels membrane expressed FasL. The blot shows that whereas FasL is detected in untreated cells lane 1 , levels increased dramatically when cells were treated with KB8301 lane 2 . A mouse IgG1 isotype matched antibody was used as a negative control lane 3 . Show More Western blot analysis of Fas Ligand FasL . L5187Y human T lymphoma cells were transfected with human FasL cDNA and then not treated lane 1 or treated lane 2 with the metalloproteinase inhibitor, KB8301. KB8301 blocks FasL cleavage from the cell surface, resulting in high levels membrane expressed FasL. The blot shows that whereas FasL is detected in untreated cells lane 1 , levels increased dramatically when cells were treated with KB8301 lane 2 . A mouse IgG1 isotype matched antibody was used as a negative control lane 3 .