BD PharmingenTM Purified Mouse Anti-Human CD25

Description

Multiparameter flow cytometric analysis of CD25 expression on unstimulated Human leucocytes Left Plots and stimulated Human lymphocytes Right Plot . Left Plots Human whole blood was stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or Purified Mouse Anti-Human CD25 antibody Cat. No. 568881; Right Plot at 0.5 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Right Plot Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-Human CD25 antibody Cat. No. 568881; solid line histogram at 0.5 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Cat. No. 550589; Multiple Adsorption . The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 expression on unstimulated Human leucocytes Left Plots and stimulated Human lymphocytes Right Plot . Left Plots Human whole blood was stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or Purified Mouse Anti-Human CD25 antibody Cat. No. 568881; Right Plot at 0.5 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Right Plot Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-Human CD25 antibody Cat. No. 568881; solid line histogram at 0.5 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Cat. No. 550589; Multiple Adsorption . The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 expression on unstimulated Human leucocytes Left Plots and stimulated Human lymphocytes Right Plot . Left Plots Human whole blood was stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or Purified Mouse Anti-Human CD25 antibody Cat. No. 568881; Right Plot at 0.5 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Right Plot Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-Human CD25 antibody Cat. No. 568881; solid line histogram at 0.5 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Cat. No. 550589; Multiple Adsorption . The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD25 expression on unstimulated Human leucocytes Left Plots and stimulated Human lymphocytes Right Plot . Left Plots Human whole blood was stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or Purified Mouse Anti-Human CD25 antibody Cat. No. 568881; Right Plot at 0.5 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Right Plot Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-Human CD25 antibody Cat. No. 568881; solid line histogram at 0.5 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Cat. No. 550589; Multiple Adsorption . The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD25 expression on unstimulated Human leucocytes Left Plots and stimulated Human lymphocytes Right Plot . Left Plots Human whole blood was stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or Purified Mouse Anti-Human CD25 antibody Cat. No. 568881; Right Plot at 0.5 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Right Plot Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-Human CD25 antibody Cat. No. 568881; solid line histogram at 0.5 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Cat. No. 550589; Multiple Adsorption . The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 expression on unstimulated Human leucocytes Left Plots and stimulated Human lymphocytes Right Plot . Left Plots Human whole blood was stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 554121; Left Plot or Purified Mouse Anti-Human CD25 antibody Cat. No. 568881; Right Plot at 0.5 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption Cat. No. 550589 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Right Plot Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA and then stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121; dashed line histogram or Purified Mouse Anti-Human CD25 antibody Cat. No. 568881; solid line histogram at 0.5 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Cat. No. 550589; Multiple Adsorption . The fluorescence histogram showing CD25 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.