BD PharmingenTM Purified Mouse Anti-Human CD276

Description

CD276 expression on human immature dendritic cells . Human PBMC were isolated using Ficoll-Paque density gradient medium and cultured for three hours. The non-adherent fraction was removed, and the remaining adherent cells were cultured with Recombinant Human IL-4 Cat. No. 554605 and Recombinant Human GM-CSF Cat. No. 550068 for five days. The cells were harvested and stained with either Purified Mouse Anti-Human CD276 Cat. No. 565828, solid-line histogram or Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121, dashed-line histogram . After washing with one time with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , the second-step reagent PE Goat Anti-Mouse Ig Cat. No. 550589 was added. Samples were washed with BD Pharmingen Stain Buffer FBS and data was acquired. The histograms were derived from gated events with the forward and side light-scatter characteristics of viable dendritic cells. Flow cytometry was performed using BD LSR II Fortessa Flow Cytometer System. Overlay plots were generated using FlowJo Software. Immunohistochemical staining of CD276 on human tonsil Top Row and placenta Bottom Row . The acetone-fixed frozen sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No.550878; Left Column or Purified Mouse Anti-Human CD276 Cat. No. 565828; Right Column . A three-step staining procedure that employed Biotin Goat Anti-Mouse Ig Cat. No. 550337 , Streptavidin HRP Cat. No. 550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. Counterstaining was with Hematoxylin. Top Row: CD276 expression can be observed on some B lymphocytes, dendritic cells and macrophages in the tonsil. Original magnification: 20. Bottom Row: CD276 expression on trophoblast and Hofbauer cells can be observed in the placenta. Original magnification: 40. CD276 expression on human immature dendritic cells . Human PBMC were isolated using Ficoll-Paque density gradient medium and cultured for three hours. The non-adherent fraction was removed, and the remaining adherent cells were cultured with Recombinant Human IL-4 Cat. No. 554605 and Recombinant Human GM-CSF Cat. No. 550068 for five days. The cells were harvested and stained with either Purified Mouse Anti-Human CD276 Cat. No. 565828, solid-line histogram or Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121, dashed-line histogram . After washing with one time with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , the second-step reagent PE Goat Anti-Mouse Ig Cat. No. 550589 was added. Samples were washed with BD Pharmingen Stain Buffer FBS and data was acquired. The histograms were derived from gated events with the forward and side light-scatter characteristics of viable dendritic cells. Flow cytometry was performed using BD LSR II Fortessa Flow Cytometer System. Overlay plots were generated using FlowJo Software. Show More Immunohistochemical staining of CD276 on human tonsil Top Row and placenta Bottom Row . The acetone-fixed frozen sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No.550878; Left Column or Purified Mouse Anti-Human CD276 Cat. No. 565828; Right Column . A three-step staining procedure that employed Biotin Goat Anti-Mouse Ig Cat. No. 550337 , Streptavidin HRP Cat. No. 550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. Counterstaining was with Hematoxylin. Top Row: CD276 expression can be observed on some B lymphocytes, dendritic cells and macrophages in the tonsil. Original magnification: 20. Bottom Row: CD276 expression on trophoblast and Hofbauer cells can be observed in the placenta. Original magnification: 40. Show More CD276 expression on human immature dendritic cells . Human PBMC were isolated using Ficoll-Paque density gradient medium and cultured for three hours. The non-adherent fraction was removed, and the remaining adherent cells were cultured with Recombinant Human IL-4 Cat. No. 554605 and Recombinant Human GM-CSF Cat. No. 550068 for five days. The cells were harvested and stained with either Purified Mouse Anti-Human CD276 Cat. No. 565828, solid-line histogram or Purified Mouse IgG1 kappa Isotype Control Cat. No. 554121, dashed-line histogram . After washing with one time with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , the second-step reagent PE Goat Anti-Mouse Ig Cat. No. 550589 was added. Samples were washed with BD Pharmingen Stain Buffer FBS and data was acquired. The histograms were derived from gated events with the forward and side light-scatter characteristics of viable dendritic cells. Flow cytometry was performed using BD LSR II Fortessa Flow Cytometer System. Overlay plots were generated using FlowJo Software. Immunohistochemical staining of CD276 on human tonsil Top Row and placenta Bottom Row . The acetone-fixed frozen sections were stained with either Purified Mouse IgG1 kappa Isotype Control Cat. No.550878; Left Column or Purified Mouse Anti-Human CD276 Cat. No. 565828; Right Column . A three-step staining procedure that employed Biotin Goat Anti-Mouse Ig Cat. No. 550337 , Streptavidin HRP Cat. No. 550946 , and the DAB Substrate Kit Cat. No. 550880 was used to develop the primary staining reagents. Counterstaining was with Hematoxylin. Top Row: CD276 expression can be observed on some B lymphocytes, dendritic cells and macrophages in the tonsil. Original magnification: 20. Bottom Row: CD276 expression on trophoblast and Hofbauer cells can be observed in the placenta. Original magnification: 40.