BD PharmingenTM Purified Mouse Anti-Human CD40

Description

Multiparameter flow cytometric analysis of CD40 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 556648; Left Plot or Purified Mouse Anti-Human CD40 antibody Cat. No. 568939; Right Plot at 0.002 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption antibody Cat. No. 550589 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD40 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 556648; Left Plot or Purified Mouse Anti-Human CD40 antibody Cat. No. 568939; Right Plot at 0.002 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption antibody Cat. No. 550589 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD40 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 556648; Left Plot or Purified Mouse Anti-Human CD40 antibody Cat. No. 568939; Right Plot at 0.002 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption antibody Cat. No. 550589 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD40 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 556648; Left Plot or Purified Mouse Anti-Human CD40 antibody Cat. No. 568939; Right Plot at 0.002 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption antibody Cat. No. 550589 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD40 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 556648; Left Plot or Purified Mouse Anti-Human CD40 antibody Cat. No. 568939; Right Plot at 0.002 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption antibody Cat. No. 550589 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD40 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either Purified Mouse IgG1, kappa Isotype Control Cat. No. 556648; Left Plot or Purified Mouse Anti-Human CD40 antibody Cat. No. 568939; Right Plot at 0.002 mug/test. The cells were then secondarily stained with PE Goat Anti-Mouse Ig Multiple Adsorption antibody Cat. No. 550589 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD40 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.